Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase

Jaldappagari Seetharamappa; Muse Oke; Huanting Liu; Stephen A McMahon; Kenneth A Johnson; Lester Carter; Mark Dorward; Michal Zawadzki; Ian M Overton; C A Johannes van Niekirk; Shirley Graham; Catherine H Botting; Garry L Taylor; Malcolm F White; Geoffrey J Barton; Peter J Coote; James H Naismith

doi:10.1107/S1744309107019562

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase

Jaldappagari Seetharamappa
, Muse Oke
, Huanting Liu
, Stephen A McMahon
, Kenneth A Johnson
, Lester Carter
, Mark Dorward
, Michal Zawadzki
, Ian M Overton
, C A Johannes van Niekirk
, Shirley Graham
, Catherine H Botting
, Garry L Taylor
, Malcolm F White
, Geoffrey J Barton
, Peter J Coote
, James H Naismith

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Original language	Undefined/Unknown
Pages (from-to)	452-6
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Volume	63
Issue number	Pt 5
DOIs	https://doi.org/10.1107/S1744309107019562
Publication status	Published - 01 May 2007

Keywords

Bacterial Proteins, Chromatography, Affinity, Cloning, Molecular, Crystallization, Methicillin Resistance, Protein Conformation, Staphylococcus aureus, Transaminases

Access to Document

10.1107/S1744309107019562

Cite this

Seetharamappa, J., Oke, M., Liu, H., McMahon, S. A., Johnson, K. A., Carter, L., Dorward, M., Zawadzki, M., Overton, I. M., van Niekirk, C. A. J., Graham, S., Botting, C. H., Taylor, G. L., White, M. F., Barton, G. J., Coote, P. J., & Naismith, J. H. (2007). Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 63(Pt 5), 452-6. https://doi.org/10.1107/S1744309107019562

Seetharamappa, Jaldappagari ; Oke, Muse ; Liu, Huanting et al. / Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase. In: Acta Crystallographica Section F Structural Biology and Crystallization Communications. 2007 ; Vol. 63, No. Pt 5. pp. 452-6.

@article{44033cd31fc94fe69624894e7da67cca,

title = "Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase",

keywords = "Bacterial Proteins, Chromatography, Affinity, Cloning, Molecular, Crystallization, Methicillin Resistance, Protein Conformation, Staphylococcus aureus, Transaminases",

author = "Jaldappagari Seetharamappa and Muse Oke and Huanting Liu and McMahon, \{Stephen A\} and Johnson, \{Kenneth A\} and Lester Carter and Mark Dorward and Michal Zawadzki and Overton, \{Ian M\} and \{van Niekirk\}, \{C A Johannes\} and Shirley Graham and Botting, \{Catherine H\} and Taylor, \{Garry L\} and White, \{Malcolm F\} and Barton, \{Geoffrey J\} and Coote, \{Peter J\} and Naismith, \{James H\}",

year = "2007",

month = may,

day = "1",

doi = "10.1107/S1744309107019562",

language = "Undefined/Unknown",

volume = "63",

pages = "452--6",

journal = "Acta Crystallographica Section F Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "Wiley-Blackwell",

number = "Pt 5",

}

Seetharamappa, J, Oke, M, Liu, H, McMahon, SA, Johnson, KA, Carter, L, Dorward, M, Zawadzki, M, Overton, IM, van Niekirk, CAJ, Graham, S, Botting, CH, Taylor, GL, White, MF, Barton, GJ, Coote, PJ & Naismith, JH 2007, 'Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase', Acta Crystallographica Section F Structural Biology and Crystallization Communications, vol. 63, no. Pt 5, pp. 452-6. https://doi.org/10.1107/S1744309107019562

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase. / Seetharamappa, Jaldappagari; Oke, Muse; Liu, Huanting et al.
In: Acta Crystallographica Section F Structural Biology and Crystallization Communications, Vol. 63, No. Pt 5, 01.05.2007, p. 452-6.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase

AU - Seetharamappa, Jaldappagari

AU - Oke, Muse

AU - Liu, Huanting

AU - McMahon, Stephen A

AU - Johnson, Kenneth A

AU - Carter, Lester

AU - Dorward, Mark

AU - Zawadzki, Michal

AU - Overton, Ian M

AU - van Niekirk, C A Johannes

AU - Graham, Shirley

AU - Botting, Catherine H

AU - Taylor, Garry L

AU - White, Malcolm F

AU - Barton, Geoffrey J

AU - Coote, Peter J

AU - Naismith, James H

PY - 2007/5/1

Y1 - 2007/5/1

KW - Bacterial Proteins, Chromatography, Affinity, Cloning, Molecular, Crystallization, Methicillin Resistance, Protein Conformation, Staphylococcus aureus, Transaminases

U2 - 10.1107/S1744309107019562

DO - 10.1107/S1744309107019562

M3 - Article

SN - 1744-3091

VL - 63

SP - 452

EP - 456

JO - Acta Crystallographica Section F Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F Structural Biology and Crystallization Communications

IS - Pt 5

ER -

Seetharamappa J, Oke M, Liu H, McMahon SA, Johnson KA, Carter L et al. Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase. Acta Crystallographica Section F Structural Biology and Crystallization Communications. 2007 May 1;63(Pt 5):452-6. doi: 10.1107/S1744309107019562