Extracellular matrix sub-types and mechanical stretch impact human cardiac fibroblast responses to transforming growth factor beta

Chris J Watson, Dermot Phelan, Patrick Collier, Stephen Horgan, Nadia Glezeva, Gordon Cooke, Maojia Xu, Mark Ledwidge, Kenneth McDonald, John A Baugh

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Understanding the impact of extracellular matrix sub-types and mechanical stretch on cardiac fibroblast activity is required to help unravel the pathophysiology of myocardial fibrotic diseases. Therefore, the purpose of this study was to investigate pro-fibrotic responses of primary human cardiac fibroblast cells exposed to different extracellular matrix components, including collagen sub-types I, III, IV, VI and laminin. The impact of mechanical cyclical stretch and treatment with transforming growth factor beta 1 (TGFβ1) on collagen 1, collagen 3 and alpha smooth muscle actin mRNA expression on different matrices was assessed using quantitative real-time PCR. Our results revealed that all of the matrices studied not only affected the expression of pro-fibrotic genes in primary human cardiac fibroblast cells at rest but also affected their response to TGFβ1. In addition, differential cellular responses to mechanical cyclical stretch were observed depending on the type of matrix the cells were adhered to. These findings may give insight into the impact of selective pathological deposition of extracellular matrix proteins within different disease states and how these could impact the fibrotic environment.

Original languageEnglish
Pages (from-to)248-256
Number of pages9
JournalConnective tissue research
Volume55
Issue number3
Early online date08 Apr 2014
DOIs
Publication statusPublished - Jun 2014

Keywords

  • Cells, Cultured
  • Collagen
  • Connective Tissue
  • Extracellular Matrix
  • Extracellular Matrix Proteins
  • Fibroblasts
  • Humans
  • Laminin
  • Stress, Mechanical
  • Transforming Growth Factor beta

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  • Cite this

    Watson, C. J., Phelan, D., Collier, P., Horgan, S., Glezeva, N., Cooke, G., Xu, M., Ledwidge, M., McDonald, K., & Baugh, J. A. (2014). Extracellular matrix sub-types and mechanical stretch impact human cardiac fibroblast responses to transforming growth factor beta. Connective tissue research, 55(3), 248-256. https://doi.org/10.3109/03008207.2014.904856