Extracellular polymeric substance-mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment

Nid'a H. Alshraiedeh; Stephen A. Kelly; Thomas P. Thompson; Padrig B. Flynn; Michael M. Tunney; Brendan F. Gilmore

doi:10.1002/ppap.202000108

Extracellular polymeric substance-mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment

Nid'a H. Alshraiedeh, Stephen A. Kelly, Thomas P. Thompson, Padrig B. Flynn, Michael M. Tunney, Brendan F. Gilmore^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

118 Downloads (Pure)

Abstract

Pseudomonas aeruginosa is an opportunistic disease-causing bacterium, with a number of strains exhibiting a mucus-forming (mucoid) phenotype during infection, producing biofilms with a surrounding matrix containing alginate. Atmospheric pressure nonthermal plasmas (APNTP) are an emerging, potential approach to control biofilms across a range of medical and industrial applications. In this study, we examine the effect of plasma treatment on P. aeruginosa biofilms from clinical samples of cystic fibrosis patients, exhibiting both mucoid and non-mucoid types. Biofilms of mucoid strains exhibit significantly elevated APNTP tolerance (p <.05). Endogenous alginate overproduction, as well as supplementation of P. aeruginosa cultures with exogenous alginate, results in significantly increased APNTP tolerance. Overall, this study shows how extracellular polymeric substance components mediate tolerance to APNTP, with significantly greater effects observed in mucoid strains.

Original language	English
Article number	2000108
Number of pages	8
Journal	Plasma Processes and Polymers
Volume	17
Issue number	12
Early online date	25 Aug 2020
DOIs	https://doi.org/10.1002/ppap.202000108
Publication status	Published - 03 Dec 2020

Keywords

alginate
biofilm
mucoid/non-mucoid
plasma
Pseudomonas aeruginosa

ASJC Scopus subject areas

Condensed Matter Physics
Polymers and Plastics

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10.1002/ppap.202000108Licence: CC BY

Extracellular polymeric substance‐mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment
Copyright 2020 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 972 KBLicence: CC BY

Cite this

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title = "Extracellular polymeric substance-mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment",

abstract = "Pseudomonas aeruginosa is an opportunistic disease-causing bacterium, with a number of strains exhibiting a mucus-forming (mucoid) phenotype during infection, producing biofilms with a surrounding matrix containing alginate. Atmospheric pressure nonthermal plasmas (APNTP) are an emerging, potential approach to control biofilms across a range of medical and industrial applications. In this study, we examine the effect of plasma treatment on P. aeruginosa biofilms from clinical samples of cystic fibrosis patients, exhibiting both mucoid and non-mucoid types. Biofilms of mucoid strains exhibit significantly elevated APNTP tolerance (p <.05). Endogenous alginate overproduction, as well as supplementation of P. aeruginosa cultures with exogenous alginate, results in significantly increased APNTP tolerance. Overall, this study shows how extracellular polymeric substance components mediate tolerance to APNTP, with significantly greater effects observed in mucoid strains.",

keywords = "alginate, biofilm, mucoid/non-mucoid, plasma, Pseudomonas aeruginosa",

author = "Alshraiedeh, {Nid'a H.} and Kelly, {Stephen A.} and Thompson, {Thomas P.} and Flynn, {Padrig B.} and Tunney, {Michael M.} and Gilmore, {Brendan F.}",

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AU - Alshraiedeh, Nid'a H.

AU - Kelly, Stephen A.

AU - Thompson, Thomas P.

AU - Flynn, Padrig B.

AU - Tunney, Michael M.

AU - Gilmore, Brendan F.

PY - 2020/12/3

Y1 - 2020/12/3

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AB - Pseudomonas aeruginosa is an opportunistic disease-causing bacterium, with a number of strains exhibiting a mucus-forming (mucoid) phenotype during infection, producing biofilms with a surrounding matrix containing alginate. Atmospheric pressure nonthermal plasmas (APNTP) are an emerging, potential approach to control biofilms across a range of medical and industrial applications. In this study, we examine the effect of plasma treatment on P. aeruginosa biofilms from clinical samples of cystic fibrosis patients, exhibiting both mucoid and non-mucoid types. Biofilms of mucoid strains exhibit significantly elevated APNTP tolerance (p <.05). Endogenous alginate overproduction, as well as supplementation of P. aeruginosa cultures with exogenous alginate, results in significantly increased APNTP tolerance. Overall, this study shows how extracellular polymeric substance components mediate tolerance to APNTP, with significantly greater effects observed in mucoid strains.

KW - alginate

KW - biofilm

KW - mucoid/non-mucoid

KW - plasma

KW - Pseudomonas aeruginosa

U2 - 10.1002/ppap.202000108

DO - 10.1002/ppap.202000108

M3 - Article

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M1 - 2000108

ER -

Extracellular polymeric substance-mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment

Abstract

Keywords

ASJC Scopus subject areas

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