Extracellular polymeric substance-mediated tolerance of Pseudomonas aeruginosa biofilms to atmospheric pressure nonthermal plasma treatment

Nid'a H. Alshraiedeh, Stephen A. Kelly, Thomas P. Thompson, Padrig B. Flynn, Michael M. Tunney, Brendan F. Gilmore*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
29 Downloads (Pure)

Abstract

Pseudomonas aeruginosa is an opportunistic disease-causing bacterium, with a number of strains exhibiting a mucus-forming (mucoid) phenotype during infection, producing biofilms with a surrounding matrix containing alginate. Atmospheric pressure nonthermal plasmas (APNTP) are an emerging, potential approach to control biofilms across a range of medical and industrial applications. In this study, we examine the effect of plasma treatment on P. aeruginosa biofilms from clinical samples of cystic fibrosis patients, exhibiting both mucoid and non-mucoid types. Biofilms of mucoid strains exhibit significantly elevated APNTP tolerance (p <.05). Endogenous alginate overproduction, as well as supplementation of P. aeruginosa cultures with exogenous alginate, results in significantly increased APNTP tolerance. Overall, this study shows how extracellular polymeric substance components mediate tolerance to APNTP, with significantly greater effects observed in mucoid strains.

Original languageEnglish
Article number2000108
Number of pages8
JournalPlasma Processes and Polymers
Volume17
Issue number12
Early online date25 Aug 2020
DOIs
Publication statusPublished - 03 Dec 2020

Keywords

  • alginate
  • biofilm
  • mucoid/non-mucoid
  • plasma
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Polymers and Plastics

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