Extraction and radioimmunoassay quantitation of neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP) from human dental pulp tissue

Ikhlas El Karim, F.T. Lundy, G.J. Linden, P.-J. Lamey

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

The measurement of neuropeptides in complex biological tissue samples requires efficient and appropriate extraction methods so that immunoreactivity is retained for subsequent radioimmunoassay detection. Since neuropeptides differ in their molecular mass, charge and hydrophobicity, no single method will suffice for the optimal extraction of various neuropeptides. In this study, dental pulp tissue was obtained from 30 human non-carious teeth. Of the three different neuropeptide extraction methods employed, boiling in acetic acid in the presence of protease inhibitors yielded the highest levels of neuropeptide Y (NPY) and vasoactive intestinal polypeptide (VIP). High pressure liquid chromatography (HPLC) analysis of dental pulp tissue verified the authenticity of the neuropeptides extracted. © 2003 Elsevier Science Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)249-254
Number of pages6
JournalArchives of Oral Biology
Volume48
Issue number3
DOIs
Publication statusPublished - 01 Mar 2003

ASJC Scopus subject areas

  • Dentistry(all)

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