Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction

Shuaimin Lu, Di Wu, Guoliang Li*, Zhengxian Lv, Peiwei Gong, Lian Xia, Zhiwei Sun, Guang Chen, Xuefeng Chen, Jinmao You, Yongning Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

The intake of N-nitrosamines (NAs) from foodstuffs is considered to be an important influence factor for several cancers. But the rapid and sensitive screening of NAs remains a challenge in the field of food safety. Inspired by that, a sensitive and rapid method was demonstrated for determination of five NAs (Nitrosopyrrolidine, Nitrosodimethylamine, Nitrosodiethylamine, Nitrosodipropylamine and Nitrosodibutylamine) using dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detection (HPLC–FLD). The NAs were firstly denitrosated and labeled by 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and finally enriched by DLLME. Furthermore, the main DLLME conditions were optimized systematically. Under the optimal conditions, satisfactory limits of detection (LODs) were obtained with a range of 0.01–0.07 ng g−1, which were significantly lower than the reported methods. The developed method showed many merits including rapidity, simplicity, high sensitivity and excellent selectivity, which shows a broad prospect in food safety analysis.

Original languageEnglish
Pages (from-to)408-415
Number of pages8
JournalFood Chemistry
Volume234
Early online date11 May 2017
DOIs
Publication statusPublished - 01 Nov 2017
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (Nos. 21677085, 21537001 and 81472986), and the Project funded by China Postdoctoral Science Foundation (No. 2016M590071).

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

  • Dispersive liquid–liquid microextraction
  • Fluorescent labeling
  • High-performance liquid chromatography
  • N-nitrosamines

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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