The intake of N-nitrosamines (NAs) from foodstuffs is considered to be an important influence factor for several cancers. But the rapid and sensitive screening of NAs remains a challenge in the field of food safety. Inspired by that, a sensitive and rapid method was demonstrated for determination of five NAs (Nitrosopyrrolidine, Nitrosodimethylamine, Nitrosodiethylamine, Nitrosodipropylamine and Nitrosodibutylamine) using dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detection (HPLC–FLD). The NAs were firstly denitrosated and labeled by 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and finally enriched by DLLME. Furthermore, the main DLLME conditions were optimized systematically. Under the optimal conditions, satisfactory limits of detection (LODs) were obtained with a range of 0.01–0.07 ng g−1, which were significantly lower than the reported methods. The developed method showed many merits including rapidity, simplicity, high sensitivity and excellent selectivity, which shows a broad prospect in food safety analysis.
|Number of pages||8|
|Early online date||11 May 2017|
|Publication status||Published - 01 Nov 2017|
Bibliographical noteFunding Information:
This work was supported by the National Natural Science Foundation of China (Nos. 21677085, 21537001 and 81472986), and the Project funded by China Postdoctoral Science Foundation (No. 2016M590071).
© 2017 Elsevier Ltd
- Dispersive liquid–liquid microextraction
- Fluorescent labeling
- High-performance liquid chromatography
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science