Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction

Shuaimin Lu; Di Wu; Guoliang Li; Zhengxian Lv; Peiwei Gong; Lian Xia; Zhiwei Sun; Guang Chen; Xuefeng Chen; Jinmao You; Yongning Wu

doi:10.1016/j.foodchem.2017.05.032

Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction

Shuaimin Lu, Di Wu, Guoliang Li^*, Zhengxian Lv, Peiwei Gong, Lian Xia, Zhiwei Sun, Guang Chen, Xuefeng Chen, Jinmao You, Yongning Wu

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

35 Citations (Scopus)

Abstract

The intake of N-nitrosamines (NAs) from foodstuffs is considered to be an important influence factor for several cancers. But the rapid and sensitive screening of NAs remains a challenge in the field of food safety. Inspired by that, a sensitive and rapid method was demonstrated for determination of five NAs (Nitrosopyrrolidine, Nitrosodimethylamine, Nitrosodiethylamine, Nitrosodipropylamine and Nitrosodibutylamine) using dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detection (HPLC–FLD). The NAs were firstly denitrosated and labeled by 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and finally enriched by DLLME. Furthermore, the main DLLME conditions were optimized systematically. Under the optimal conditions, satisfactory limits of detection (LODs) were obtained with a range of 0.01–0.07 ng g⁻¹, which were significantly lower than the reported methods. The developed method showed many merits including rapidity, simplicity, high sensitivity and excellent selectivity, which shows a broad prospect in food safety analysis.

Original language	English
Pages (from-to)	408-415
Number of pages	8
Journal	Food Chemistry
Volume	234
Early online date	11 May 2017
DOIs	https://doi.org/10.1016/j.foodchem.2017.05.032
Publication status	Published - 01 Nov 2017
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (Nos. 21677085, 21537001 and 81472986), and the Project funded by China Postdoctoral Science Foundation (No. 2016M590071).

Publisher Copyright:
© 2017 Elsevier Ltd

Keywords

Dispersive liquid–liquid microextraction
Fluorescent labeling
High-performance liquid chromatography
N-nitrosamines

ASJC Scopus subject areas

Analytical Chemistry
Food Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

Lu, S., Wu, D., Li, G., Lv, Z., Gong, P., Xia, L., Sun, Z., Chen, G., Chen, X., You, J., & Wu, Y. (2017). Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction. Food Chemistry, 234, 408-415. https://doi.org/10.1016/j.foodchem.2017.05.032

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title = "Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction",

abstract = "The intake of N-nitrosamines (NAs) from foodstuffs is considered to be an important influence factor for several cancers. But the rapid and sensitive screening of NAs remains a challenge in the field of food safety. Inspired by that, a sensitive and rapid method was demonstrated for determination of five NAs (Nitrosopyrrolidine, Nitrosodimethylamine, Nitrosodiethylamine, Nitrosodipropylamine and Nitrosodibutylamine) using dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detection (HPLC–FLD). The NAs were firstly denitrosated and labeled by 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and finally enriched by DLLME. Furthermore, the main DLLME conditions were optimized systematically. Under the optimal conditions, satisfactory limits of detection (LODs) were obtained with a range of 0.01–0.07 ng g−1, which were significantly lower than the reported methods. The developed method showed many merits including rapidity, simplicity, high sensitivity and excellent selectivity, which shows a broad prospect in food safety analysis.",

keywords = "Dispersive liquid–liquid microextraction, Fluorescent labeling, High-performance liquid chromatography, N-nitrosamines",

author = "Shuaimin Lu and Di Wu and Guoliang Li and Zhengxian Lv and Peiwei Gong and Lian Xia and Zhiwei Sun and Guang Chen and Xuefeng Chen and Jinmao You and Yongning Wu",

note = "Funding Information: This work was supported by the National Natural Science Foundation of China (Nos. 21677085, 21537001 and 81472986), and the Project funded by China Postdoctoral Science Foundation (No. 2016M590071). Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

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Lu, S, Wu, D, Li, G, Lv, Z, Gong, P, Xia, L, Sun, Z, Chen, G, Chen, X, You, J & Wu, Y 2017, 'Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction', Food Chemistry, vol. 234, pp. 408-415. https://doi.org/10.1016/j.foodchem.2017.05.032

TY - JOUR

T1 - Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction

AU - Lu, Shuaimin

AU - Wu, Di

AU - Li, Guoliang

AU - Lv, Zhengxian

AU - Gong, Peiwei

AU - Xia, Lian

AU - Sun, Zhiwei

AU - Chen, Guang

AU - Chen, Xuefeng

AU - You, Jinmao

AU - Wu, Yongning

N1 - Funding Information: This work was supported by the National Natural Science Foundation of China (Nos. 21677085, 21537001 and 81472986), and the Project funded by China Postdoctoral Science Foundation (No. 2016M590071). Publisher Copyright: © 2017 Elsevier Ltd

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The intake of N-nitrosamines (NAs) from foodstuffs is considered to be an important influence factor for several cancers. But the rapid and sensitive screening of NAs remains a challenge in the field of food safety. Inspired by that, a sensitive and rapid method was demonstrated for determination of five NAs (Nitrosopyrrolidine, Nitrosodimethylamine, Nitrosodiethylamine, Nitrosodipropylamine and Nitrosodibutylamine) using dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detection (HPLC–FLD). The NAs were firstly denitrosated and labeled by 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and finally enriched by DLLME. Furthermore, the main DLLME conditions were optimized systematically. Under the optimal conditions, satisfactory limits of detection (LODs) were obtained with a range of 0.01–0.07 ng g−1, which were significantly lower than the reported methods. The developed method showed many merits including rapidity, simplicity, high sensitivity and excellent selectivity, which shows a broad prospect in food safety analysis.

AB - The intake of N-nitrosamines (NAs) from foodstuffs is considered to be an important influence factor for several cancers. But the rapid and sensitive screening of NAs remains a challenge in the field of food safety. Inspired by that, a sensitive and rapid method was demonstrated for determination of five NAs (Nitrosopyrrolidine, Nitrosodimethylamine, Nitrosodiethylamine, Nitrosodipropylamine and Nitrosodibutylamine) using dispersive liquid–liquid microextraction (DLLME) followed by high-performance liquid chromatography with fluorescence detection (HPLC–FLD). The NAs were firstly denitrosated and labeled by 2-(11H-benzo[a]carbazol-11-yl) ethyl carbonochloridate (BCEC-Cl) and finally enriched by DLLME. Furthermore, the main DLLME conditions were optimized systematically. Under the optimal conditions, satisfactory limits of detection (LODs) were obtained with a range of 0.01–0.07 ng g−1, which were significantly lower than the reported methods. The developed method showed many merits including rapidity, simplicity, high sensitivity and excellent selectivity, which shows a broad prospect in food safety analysis.

KW - Dispersive liquid–liquid microextraction

KW - Fluorescent labeling

KW - High-performance liquid chromatography

KW - N-nitrosamines

U2 - 10.1016/j.foodchem.2017.05.032

DO - 10.1016/j.foodchem.2017.05.032

M3 - Article

C2 - 28551254

AN - SCOPUS:85019163778

VL - 234

SP - 408

EP - 415

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

ER -

Facile and sensitive determination of N-nitrosamines in food samples by high-performance liquid chromatography via combining fluorescent labeling with dispersive liquid-liquid microextraction

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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