Facile fabrication of highly quantum Dot/AuNP-loaded tags for a dual-modal colorimetric/reversed ratiometric fluorescence immunochromatographic assay

Tian Guan, Yudong Shen, Zhuo Jiang, Jin Wang, Shiwei Zhang, Anastasios Koidis, Xiaojun Yao, Yiyong Yan, Hongtao Lei*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
111 Downloads (Pure)

Abstract

Developing an easily-prepared, sensitive, and accurate point-of-need immunochromatographic assay (ICA) is significant in food safety screening, clinical diagnosis, and environmental monitoring. However, the current single-modal ICAs are limited in certain instinct drawbacks that restrict analytical performances. Herein, we introduce an ultrasensitive dual-modal colorimetric/reversed ratiometric fluorescence ICA based on facilely prepared immunoprobes with a high loading capacity of red quantum dots and AuNPs. By smartly integrating these red-colored/fluorescent signal probes with an immobilized green quantum dot antigen on the test lines, discrete "turn-on"visual inspection and reversed ratiometric quantification via a portable smartphone-based analyzer were accomplished. As an application, this method was employed to detect 11 phosphodiesterase-5 inhibitors in health foods with ultralow detection limits (0.0028-0.045 ng/mL), high repeatability (coefficient of variations of 0.3-1.91%), and reasonable accuracy (recoveries of 86.6-107%). The proposed method was further validated by the authorized liquid chromatography with tandem mass spectrometry method in actual sample detection. This new assay format can be extended to ultrasensitive flexible detection of other food contaminants, environmental pollutants, or tumor biomarkers within minutes, and it just requires simply prepared signal reporters, easy-to-operate procedures, and a low-cost miniaturized analyzer.

Original languageEnglish
Pages (from-to)13463–13472
JournalAnalytical Chemistry
Volume94
Issue number39
Early online date21 Sept 2022
DOIs
Publication statusPublished - 04 Oct 2022

Bibliographical note

Funding Information:
This work was financially supported by the National Natural Science Foundation of China (31871883 and 21874048), He Yuan Planned Program in Science and Technology (2019041), Generic Technique Innovation Team Construction of Modern Agriculture of Guangdong Province (2022KJ130, 2023KJ130), National Key Research and Development Program of Thirteenth Five-Year Plan (2017YFC1601700), Guangdong Basic and Applied Basic Research Foundation (2019B1515210025 and 2020A1515011238), and Science and Technology Planning Project of the Guangxi (AB18126048).

Publisher Copyright:
© 2022 American Chemical Society.

ASJC Scopus subject areas

  • Analytical Chemistry

Fingerprint

Dive into the research topics of 'Facile fabrication of highly quantum Dot/AuNP-loaded tags for a dual-modal colorimetric/reversed ratiometric fluorescence immunochromatographic assay'. Together they form a unique fingerprint.

Cite this