FACT facilitates chromatin transcription by RNA polymerases I and III

J.L. Birch, B.C. Tan, Konstantin Panov, T.B. Panova, J.S. Andersen, T.A. Owen-Hughes, J. Russell, S.C. Lee, J.C. Zomerdijk

Research output: Contribution to journalArticle

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Abstract

Efficient transcription elongation from a chromatin template requires RNA polymerases (Pols) to negotiate nucleosomes. Our biochemical analyses demonstrate that RNA Pol I can transcribe through nucleosome templates and that this requires structural rearrangement of the nucleosomal core particle. The subunits of the histone chaperone FACT (facilitates chromatin transcription), SSRP1 and Spt16, co-purify and co-immunoprecipitate with mammalian Pol I complexes. In cells, SSRP1 is detectable at the rRNA gene repeats. Crucially, siRNA-mediated repression of FACT subunit expression in cells results in a significant reduction in 47S pre-rRNA levels, whereas synthesis of the first 40 nt of the rRNA is not affected, implying that FACT is important for Pol I transcription elongation through chromatin. FACT also associates with RNA Pol III complexes, is present at the chromatin of genes transcribed by Pol III and facilitates their transcription in cells. Our findings indicate that, beyond the established role in Pol II transcription, FACT has physiological functions in chromatin transcription by all three nuclear RNA Pols. Our data also imply that local chromatin dynamics influence transcription of the active rRNA genes by Pol I and of Pol III-transcribed genes.
LanguageEnglish
Pages854-865
Number of pages12
JournalThe EMBO Journal
Volume28
Issue number7
Early online date12 Feb 2009
DOIs
Publication statusPublished - 08 Apr 2009

Fingerprint

RNA Polymerase I
RNA Polymerase III
Transcription
Chromatin
Genes
Nucleosomes
DNA-Directed RNA Polymerases
rRNA Genes
Histone Chaperones
Elongation
pol Genes
Nuclear RNA
RNA
RNA Precursors
Small Interfering RNA

Cite this

Birch, J. L., Tan, B. C., Panov, K., Panova, T. B., Andersen, J. S., Owen-Hughes, T. A., ... Zomerdijk, J. C. (2009). FACT facilitates chromatin transcription by RNA polymerases I and III. The EMBO Journal, 28(7), 854-865. https://doi.org/10.1038/emboj.2009.33
Birch, J.L. ; Tan, B.C. ; Panov, Konstantin ; Panova, T.B. ; Andersen, J.S. ; Owen-Hughes, T.A. ; Russell, J. ; Lee, S.C. ; Zomerdijk, J.C. / FACT facilitates chromatin transcription by RNA polymerases I and III. In: The EMBO Journal. 2009 ; Vol. 28, No. 7. pp. 854-865.
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Birch, JL, Tan, BC, Panov, K, Panova, TB, Andersen, JS, Owen-Hughes, TA, Russell, J, Lee, SC & Zomerdijk, JC 2009, 'FACT facilitates chromatin transcription by RNA polymerases I and III', The EMBO Journal, vol. 28, no. 7, pp. 854-865. https://doi.org/10.1038/emboj.2009.33

FACT facilitates chromatin transcription by RNA polymerases I and III. / Birch, J.L.; Tan, B.C.; Panov, Konstantin; Panova, T.B.; Andersen, J.S.; Owen-Hughes, T.A.; Russell, J.; Lee, S.C.; Zomerdijk, J.C.

In: The EMBO Journal, Vol. 28, No. 7, 08.04.2009, p. 854-865.

Research output: Contribution to journalArticle

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Birch JL, Tan BC, Panov K, Panova TB, Andersen JS, Owen-Hughes TA et al. FACT facilitates chromatin transcription by RNA polymerases I and III. The EMBO Journal. 2009 Apr 8;28(7):854-865. https://doi.org/10.1038/emboj.2009.33