Family-based investigation of the C677T polymorphism of the methylenetetrahydrofolate reductase gene in ischaemic heart disease

M.S. Spence, P.G. McGlinchey, Christopher Patterson, Christine Belton, G. Murphy, D. McMaster, Damian Fogarty, Alun Evans, Pascal McKeown

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Elevated homocysteine is associated with ischaemic heart disease (IHD). The C677T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene results in reduced MTHFR enzyme activity and reduced methylation of homocysteine to methionine resulting in mild hyperhomocysteinaemia. Case-control association studies of the role of the C677T MTHFR polymorphism in IHD have produced conflicting results. We therefore used newly described family-based association tests to investigate the role of this polymorphism in IHD, in a well-defined population. Methods: A total of 352 individuals from 129 families (discordant sibships and parent-child trios) were recruited. Linkage disequilibrium between the polymorphism and IHD was tested for using the combined transmission disequilibrium test (TDT)/sib-TDT and pedigree disequilibrium test (PDT). Homocysteine levels were measured. Results: Both the TDT/sib-TDT and PDT analyses found a significantly reduced transmission of the T allele to affected individuals (P=0.016 and P=0.021). There was no significant difference in homocysteine levels between affected and unaffected siblings. TT homozygotes had mean homocysteine levels significantly higher than those of TC heterozygotes (P
Original languageEnglish
Pages (from-to)293-299
Number of pages7
JournalAtherosclerosis
Volume165(2)
Issue number2
DOIs
Publication statusPublished - 01 Dec 2002

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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