FASCIOLA-HEPATICA - LOCALIZATION AND PARTIAL CHARACTERIZATION OF TUBULIN

Alan Stitt, Ian Fairweather, Alan Trudgett, C.F. Johnston

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

The localisation and distribution of the cytoskeletal protein tubulin in the adult liver fluke Fasciola hepatica have been determined by an indirect immunofluorescence technique using a monoclonal antibody raised against beta-tubulin. Tubulin was demonstrated in the tegumental syncytium and in the tegumental cell bodies and their cytoplasmic connections with the surface syncytium. Immunostaining was also evident in the nerve fibres innervating sensory receptors in the tegument, in the nerve plexus innervating the sub-tegumental musculature and in the cytoplasmic extensions of the nurse cells within the vitelline follicle. Immunoblotting of whole fluke fractions produced a single band corresponding to a molecule of approximately 54 kDa in size. This figure corresponds with previous data obtained on tubulin from other helminth and eukaryotic sources.
Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalParasitology Research
Volume78
Publication statusPublished - 1992

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