Fine mapping of genes determining extrafusal fiber properties in murine soleus muscle

Andrew Carroll, Riyang Cheng, Elaina Collie-Duguid, Caroline Meharg, Michael Scholz, Stephen Fiering, Jennifer Fields, Abraham Palmer, Arimantas Lionikas

Research output: Contribution to journalArticle

6 Citations (Scopus)
199 Downloads (Pure)

Abstract

Muscle fiber cross-sectional area (CSA) and proportion of different fiber types are important determinants of muscle function and overall metabolism. Genetic variation plays a substantial role in phenotypic variation of these traits; however, the underlying genes remain poorly understood. This study aimed to map quantitative trait loci (QTL) affecting differences in soleus muscle fiber traits between the LG/J and SM/J mouse strains. Fiber number, CSA, and proportion of oxidative type I fibers were assessed in the soleus of 334 genotyped female and male mice of the F34generation of advanced intercross lines (AIL) derived from the LG/J and SM/J strains. To increase the QTL detection power, these data were combined with 94 soleus samples from the F2intercross of the same strains. Transcriptome of the soleus muscle of LG/J and SM/J females was analyzed by microarray. Genome-wide association analysis mapped four QTL (genome-wide P < 0.05) affecting the properties of muscle fibers to chromosome 2, 3, 4, and 11. A 1.5-LOD QTL support interval ranged between 2.36 and 4.67 Mb. On the basis of the genomic sequence information and functional and transcriptome data, we identified candidate genes for each of these QTL. The combination of analyses in F2 and F34 AIL populations with transcriptome and genomic sequence data in the parental strains is an effective strategy for refining QTL and nomination of the candidate genes.
Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalPHYSIOLOGICAL GENOMICS
Volume49
Issue number3
DOIs
Publication statusPublished - 01 Mar 2017

Fingerprint Dive into the research topics of 'Fine mapping of genes determining extrafusal fiber properties in murine soleus muscle'. Together they form a unique fingerprint.

  • Cite this

    Carroll, A., Cheng, R., Collie-Duguid, E., Meharg, C., Scholz, M., Fiering, S., Fields, J., Palmer, A., & Lionikas, A. (2017). Fine mapping of genes determining extrafusal fiber properties in murine soleus muscle. PHYSIOLOGICAL GENOMICS, 49(3), 141-150. https://doi.org/10.1152/physiolgenomics.00092.2016