First report of a novel plant lysozyme with both antifungal and antibacterial activities.

S. Wang, T. Ng, Tianbao Chen, D. Lin, J. Wu, P. Rao, X. Ye

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

A novel lysozyme exhibiting antifungal activity and with a molecular mass of 14.4 kDa in SDS–polyacrylamide gel electrophoresis was isolated from mung bean (Phaseolus mungo) seeds using a procedure that involved aqueous extraction, ammonium sulfate precipitation, ion exchange chromatography on CM-Sephadex, and high-performance liquid chromatography on POROS HS-20. Its N-terminal sequence was very different from that of hen egg white lysozyme. Its pI was estimated to be above 9.7. The specific activity of the lysozyme was 355 U/mg at pH 5.5 and 30 °C. The lysozyme exhibited a pH optimum at pH 5.5 and a temperature optimum at 55 °C. It is reported herein, for the first time, that a novel plant lysozyme exerted an antifungal action toward Fusarium oxysporum, Fusarium solani, Pythium aphanidermatum, Sclerotium rolfsii, and Botrytis cinerea, in addition to an antibacterial action against Staphylococcus aureus.
Original languageEnglish
Pages (from-to)820-827
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume327 (3)
Issue number3
DOIs
Publication statusPublished - 18 Feb 2005

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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