Fluorescence (FISH) and chromogenic (CISH) in situ hybridisation in prostate carcinoma cell lines: comparison and use of virtual microscopy

Kathryn Louise Elliott, Peter Hamilton, Pamela Maxwell

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Chromogenic in situ hybridisation (CISH) has become an attractive alternative to fluorescence in situ hybridisation (FISH) due to its permanent stain which is more familiar to pathologists and because it can be viewed using light microscopy, The aim of the present study is to examine reproducibility in the assessment of abnormal chromosome number by CISH in comparison to FISH. Using three prostate cell lines - PNTIA (derived from normal epithelium), LNCAP and DU145 (derived from prostatic carcinoma), chromosomes 7 and 8 were counted in 40 nuclei in FISH preparations (x100 oil immersion) and 100 nuclei in CISH preparations (x40) by two independent observers. The CISH slides were examined using standard fight microscopy and virtual microscopy. Reproducibitity was examined using paired Student's t-test (P
Original languageEnglish
Pages (from-to)167-171
Number of pages5
JournalBritish Journal of Biomedical Science
Volume65
Issue number4
Publication statusPublished - 2008

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical
  • Immunology
  • Microbiology
  • Immunology and Allergy
  • Infectious Diseases
  • Microbiology (medical)

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