Foxp3+ T cells induce Perforin-Dependent Dendritic Cell Death in Tumor-Draining Lymph Nodes.

A. Boissonnas, A. Scholer-Dahirel, V. Simon-Blancal, L. Pace, F. Valet, Adrien Kissenpfennig, T. Sparwasser, B. Malissen, L. Fetler, S. Amigorena

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)


Regulatory T (Treg) cells limit the onset of effective antitumor immunity, through yet-ill-defined mechanisms. We showed the rejection of established ovalbumin (OVA)-expressing MCA101 tumors required both the adoptive transfer of OVA-specific CD8(+) T cell receptor transgenic T cells (OTI) and the neutralization of Foxp3(+) T cells. In tumor-draining lymph nodes, Foxp3(+) T cell neutralization induced a marked arrest in the migration of OTI T cells, increased numbers of dendritic cells (DCs), and enhanced OTI T cell priming. Using an in vitro cytotoxic assay and two-photon live microscopy after adoptive transfer of DCs, we demonstrated that Foxp3(+) T cells induced the death of DCs in tumor-draining lymph nodes, but not in the absence of tumor. DC death correlated with Foxp3(+) T cell-DC contacts, and it was tumor-antigen and perforin dependent. We conclude that Foxp3(+) T cell-dependent DC death in tumor-draining lymph nodes limits the onset of CD8(+) T cell responses.
Original languageEnglish
Pages (from-to)266-278
Number of pages13
Issue number2
Publication statusPublished - 26 Feb 2010

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Infectious Diseases


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