Functional characterization of SjB10, an intracellular serpin from Schistosoma japonicum

Adebayo J Molehin, Geoffrey N Gobert, Patrick Driguez, Donald P McManus

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Serine protease inhibitors (serpin) play essential roles in many organisms. Mammalian serpins regulate the blood coagulation, fibrinolysis, inflammation and complement activation pathways. In parasitic helminths, serpins are less well characterized, but may also be involved in evasion of the host immune response. In this study, a Schistosoma japonicum serpin (SjB10), containing a 1212 bp open reading frame (ORF), was cloned, expressed and functionally characterized. Sequence analysis, comparative modelling and structural-based alignment revealed that SjB10 contains the essential structural motifs and consensus secondary structures of inhibitory serpins. Transcriptional profiling demonstrated that SjB10 is expressed in adult males, schistosomula and eggs but particularly in the cercariae, suggesting a possible role in cercarial penetration of mammalian host skin. Recombinant SjB10 (rSjB10) inhibited pancreatic elastase (PE) in a dose-dependent manner. rSjB10 was recognized strongly by experimentally infected rat sera indicating that native SjB10 is released into host tissue and induces an immune response. By immunochemistry, SjB10 localized in the S. japonicum adult foregut and extra-embryonic layer of the egg. This study provides a comprehensive demonstration of sequence and structural-based analysis of a functional S. japonicum serpin. Furthermore, our findings suggest that SjB10 may be associated with important functional roles in S. japonicum particularly in host-parasite interactions.
Original languageEnglish
Pages (from-to)1746-1760
JournalParasitology
Volume141
Issue number13
Early online date19 Aug 2014
DOIs
Publication statusPublished - Nov 2014

Keywords

  • Amino Acid Sequence
  • Animals
  • Cercaria
  • DNA, Complementary
  • Female
  • Helminth Proteins
  • Host-Parasite Interactions
  • Intracellular Space
  • Male
  • Molecular Sequence Data
  • Open Reading Frames
  • Phylogeny
  • Rats
  • Rats, Wistar
  • Schistosoma japonicum
  • Sequence Alignment
  • Serine Proteinase Inhibitors
  • Serpins

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