Functional Consequences of Splicing of the Antisense Transcript COOLAIR on FLC Transcription

Sebastain Marquardt, Oleg Raitskin, Zhe Wu, Fuquan Liu, Qianwen Sun, Caroline Dean

Research output: Contribution to journalArticle

118 Citations (Scopus)
281 Downloads (Pure)

Abstract

Antisense transcription is widespread in many genomes; however, how much is functional is hotly debated. We are investigating functionality of a set of long noncoding antisense transcripts, collectively called COOLAIR, produced at Arabidopsis FLOWERING LOCUS C (FLC). COOLAIR initiates just downstream of the major sense transcript poly(A) site and terminates either early or extends into the FLC promoter region. We now show that splicing of COOLAIR is functionally important. This was revealed through analysis of a hypomorphic mutation in the core spliceosome component PRP8. The prp8 mutation perturbs a cotranscriptional feedback mechanism linking COOLAIR processing to FLC gene body histone demethylation and reduced FLC transcription. The importance of COOLAIR splicing in this repression mechanism was confirmed by disrupting COOLAIR production and mutating the COOLAIR proximal splice acceptor site. Our findings suggest that altered splicing of a long noncoding transcript can quantitatively modulate gene expression through cotranscriptional coupling mechanisms.
Original languageEnglish
Pages (from-to)156-165
Number of pages10
JournalMolecular Cell
Volume54
Issue number1
DOIs
Publication statusPublished - 10 Apr 2014

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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