Gamma ray-induced bystander effect in tumour glioblastoma cells: a specific study on cell survival, cytokine release and cytokine receptors.

A. Facoetti, F. Ballerini, R. Cherubini, S. Gerardi, R. Nano, A. Ottolenghi, Kevin Prise, K.R. Trott, C. Zilio

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Recent experimental evidence has challenged the paradigm according to which radiation traversal through the nucleus of a cell is a prerequisite for producing genetic changes or biological responses. Thus, unexposed cells in the vicinity of directly irradiated cells or recipient cells of medium from irradiated cultures can also be affected. The aim of the present study was to evaluate, by means of the medium transfer technique, whether interleukin-8 and its receptor (CXCR1) may play a role in the bystander effect after gamma irradiation of T98G cells in vitro. In fact the cell specificity in inducing the bystander effect and in receiving the secreted signals that has been described suggests that not only the ability to release the cytokines but also the receptor profiles are likely to modulate the cell responses and the final outcome. The dose and time dependence of the cytokine release into the medium, quantified using an enzyme linked immunosorbent assay, showed that radiation causes alteration in the release of interleukin-8 from exposed cells in a dose-independent but time-dependent manner. The relative receptor expression was also affected in exposed and bystander cells.
Original languageEnglish
Pages (from-to)271-274
Number of pages4
JournalRadiation Protection Dosimetry
Volume122(1-4)
Issue number1-4
DOIs
Publication statusPublished - Dec 2006

ASJC Scopus subject areas

  • Nuclear Energy and Engineering
  • Radiology Nuclear Medicine and imaging
  • Radiation
  • Radiological and Ultrasound Technology

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