Generation of truncated derivatives through in silico enzymatic digest of peptide GV30 target MRSA both in vitro and in vivo

Yingxue Ma, Aifang Yao, Xiaoling Chen, Lei Wang, Chengbang Ma, Xinping Xi, Tianbao Chen, Chris Shaw, Mei Zhou

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
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Methicillin-resistant (MRSA) causing serious hospital-acquired infections and skin infections has become a "superbug" in clinical treatment. Although the clinical treatment of MRSA is continuously improving, due to its unceasing global spread, MRSA has produced much heated discussion and focused study, therefore suggesting an urgent task to find new antibacterial drugs to combat this issue. Antimicrobial peptides (AMPs) are used as the last-resort drugs for treating multidrug-resistant bacterial infections, but their utilisation is still limited due to their low stability and often strong toxicity. Here, we evaluated the structure and the bioactivity of an AMP, GV30, derived from the frog skin secretions of and designed seven truncated derivatives based on the presence of cleavage sites for trypsin using an online proteomic bioinformatic resource PeptideCutter tool. We investigated the anti-MRSA effect, toxicity and salt- and serum-resistance of these peptides. Interestingly, the structure-activity relationship revealed that removing "Rana box" loop could significantly improve the bactericidal speed on MRSA. Among these derivatives, GV21 (GVIFNALKGVAKTVAAQLLKK-NH ), because of its faster antibacterial effect, lower toxicity, and retains the good antibacterial activity and stability of the parent peptide, is considered to become a new potential antibacterial candidate against MRSA.
Original languageEnglish
Pages (from-to)4984-4996
JournalComputational and Structural Biotechnology Journal
Early online date29 Aug 2021
Publication statusEarly online date - 29 Aug 2021


  • Antibacterial effect
  • Antimicrobial peptides
  • Methicillin-resistant Staphylococcus aureus
  • PeptideCutter tool
  • Salt- and serum-resistance
  • Toxicity
  • Truncated derivatives


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