Genetic strategies to understand human diabetic nephropathy: wet-lab approaches

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy, biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next generation sequencing data to identify genetic risk factors for diabetic nephropathy.
Original languageEnglish
Title of host publicationMethods in Molecular Biology
Subtitle of host publicationMolecular Methods in Diabetic Nephropathy
PublisherSpringer Nature
Pages205-240
Number of pages26
Volume2067
DOIs
Publication statusPublished - 01 Jan 2020

Fingerprint

Diabetic Nephropathies
Semiconductors
Computer Simulation
Ions
Phenotype

Cite this

Smyth, L., Kerr, K., Duffy, S., Kilner, J., & McKnight, A. (2020). Genetic strategies to understand human diabetic nephropathy: wet-lab approaches. In Methods in Molecular Biology: Molecular Methods in Diabetic Nephropathy (Vol. 2067, pp. 205-240). Springer Nature. https://doi.org/10.1007/978-1-4939-9841-8_15
Smyth, Laura ; Kerr, Katie ; Duffy, Seamus ; Kilner, Jill ; McKnight, Amy. / Genetic strategies to understand human diabetic nephropathy: wet-lab approaches. Methods in Molecular Biology: Molecular Methods in Diabetic Nephropathy. Vol. 2067 Springer Nature, 2020. pp. 205-240
@inbook{24a3fc9362e4403aa4a0dc0f17e7317a,
title = "Genetic strategies to understand human diabetic nephropathy: wet-lab approaches",
abstract = "Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy, biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next generation sequencing data to identify genetic risk factors for diabetic nephropathy.",
author = "Laura Smyth and Katie Kerr and Seamus Duffy and Jill Kilner and Amy McKnight",
year = "2020",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-9841-8_15",
language = "English",
volume = "2067",
pages = "205--240",
booktitle = "Methods in Molecular Biology",
publisher = "Springer Nature",
address = "United States",

}

Smyth, L, Kerr, K, Duffy, S, Kilner, J & McKnight, A 2020, Genetic strategies to understand human diabetic nephropathy: wet-lab approaches. in Methods in Molecular Biology: Molecular Methods in Diabetic Nephropathy. vol. 2067, Springer Nature, pp. 205-240. https://doi.org/10.1007/978-1-4939-9841-8_15

Genetic strategies to understand human diabetic nephropathy: wet-lab approaches. / Smyth, Laura; Kerr, Katie; Duffy, Seamus; Kilner, Jill; McKnight, Amy.

Methods in Molecular Biology: Molecular Methods in Diabetic Nephropathy. Vol. 2067 Springer Nature, 2020. p. 205-240.

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

TY - CHAP

T1 - Genetic strategies to understand human diabetic nephropathy: wet-lab approaches

AU - Smyth, Laura

AU - Kerr, Katie

AU - Duffy, Seamus

AU - Kilner, Jill

AU - McKnight, Amy

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy, biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next generation sequencing data to identify genetic risk factors for diabetic nephropathy.

AB - Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy, biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next generation sequencing data to identify genetic risk factors for diabetic nephropathy.

U2 - 10.1007/978-1-4939-9841-8_15

DO - 10.1007/978-1-4939-9841-8_15

M3 - Chapter (peer-reviewed)

VL - 2067

SP - 205

EP - 240

BT - Methods in Molecular Biology

PB - Springer Nature

ER -

Smyth L, Kerr K, Duffy S, Kilner J, McKnight A. Genetic strategies to understand human diabetic nephropathy: wet-lab approaches. In Methods in Molecular Biology: Molecular Methods in Diabetic Nephropathy. Vol. 2067. Springer Nature. 2020. p. 205-240 https://doi.org/10.1007/978-1-4939-9841-8_15