Genetic strategies to understand human diabetic nephropathy: wet-lab approaches

Laura Smyth; Katie Kerr; Seamus Duffy; Jill Kilner; Amy McKnight

doi:10.1007/978-1-4939-9841-8_15

Genetic strategies to understand human diabetic nephropathy: wet-lab approaches

Laura Smyth, Katie Kerr, Seamus Duffy, Jill Kilner, Amy McKnight

School of Medicine, Dentistry and Biomedical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

1 Citation (Scopus)

Abstract

Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy, biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next generation sequencing data to identify genetic risk factors for diabetic nephropathy.

Original language	English
Title of host publication	Methods in Molecular Biology
Subtitle of host publication	Molecular Methods in Diabetic Nephropathy
Publisher	Springer Nature US
Pages	205-240
Number of pages	26
Volume	2067
DOIs	https://doi.org/10.1007/978-1-4939-9841-8_15
Publication status	Published - 01 Jan 2020

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10.1007/978-1-4939-9841-8_15Licence: Unspecified

R3789CPH: Discovery of an integrated risk profile for chronic kidney disease and development of a clinical biomarker panel for personalising medicine (Stratified medicine for chronic kidney disease)
McKnight, A. J. (PI) & Maxwell, P. (CoI)
15/06/2016 → 31/12/2023
Project: Research

Cite this

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abstract = "Multiple genetic strategies are available to help improve understanding of diabetic nephropathy. This methods chapter provides an overview of phenotype considerations specific to diabetic nephropathy, biobank essentials, and provides detailed methodology for a common benchtop wet-lab approach (Ion Torrent semiconductor sequencing) including in silico genetic variant identification from next generation sequencing data to identify genetic risk factors for diabetic nephropathy.",

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Genetic strategies to understand human diabetic nephropathy: wet-lab approaches

Abstract

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Projects

R3789CPH: Discovery of an integrated risk profile for chronic kidney disease and development of a clinical biomarker panel for personalising medicine (Stratified medicine for chronic kidney disease)

Cite this