Genome-wide experimental determination of barriers to horizontal gene transfer

Rotem Sorek, Yiwen Zhu, Christopher J. Creevey, M. Pilar Francino, Peer Bork, Edward M. Rubin*

*Corresponding author for this work

Research output: Contribution to journalArticle

255 Citations (Scopus)

Abstract

Horizontal gene transfer, in which genetic material is transferred from the genome of one organism to that of another, has been investigated in microbial species mainly through computational sequence analyses. To address the lack of experimental data, we studied the attempted movement of 246,045 genes from 79 prokaryotic genomes into Escherichia coli and identified genes that consistently fail to transfer. We studied the mechanisms underlying transfer inhibition by placing coding regions from different species under the control of inducible promoters. Our data suggest that toxicity to the host inhibited transfer regardless of the species of origin and that increased gene dosage and associated increased expression may be a predominant cause for transfer failure. Although these experimental studies examined transfer solely into E. coli, a computational analysis of gene-transfer rates across available bacterial and archaeal genomes supports that the barriers observed in our study are general across the tree of life.

Original languageEnglish
Pages (from-to)1449-1452
Number of pages4
JournalScience
Volume318
Issue number5855
DOIs
Publication statusPublished - 30 Nov 2007
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Sorek, R., Zhu, Y., Creevey, C. J., Francino, M. P., Bork, P., & Rubin, E. M. (2007). Genome-wide experimental determination of barriers to horizontal gene transfer. Science, 318(5855), 1449-1452. https://doi.org/10.1126/science.1147112