Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions

Leonid Kulakov; V.N. Ksenzenko; M.G. Shlyapnikov; V.V. Kochetkov; Antonio Del Casale; Christopher Allen; Mike Larkin; P- Ceyssens; R. Lavigne

doi:10.1016/j.virol.2009.06.024

Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions

Leonid Kulakov, V.N. Ksenzenko, M.G. Shlyapnikov, V.V. Kochetkov, Antonio Del Casale, Christopher Allen, Mike Larkin, P- Ceyssens, R. Lavigne

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The "phiKMV-like viruses" comprise an important genus of T7 related phages infecting Pseudomonas aeruginosa. The genomes of these bacteriophages have localized single-strand interruptions (nicks), a distinguishing genomic trait previously thought to be unique for T5 related coliphages. Analysis of this feature in the newly sequenced phage fkF77 shows all four nicks to be localized on the non-coding DNA strand. They are present with high frequencies within the phage population and are introduced into the phage DNA at late stages of the lytic cycle. The general consensus sequence in the nicks (5'-CGACxxxxxCCTAoh pCTCCGG-3') was shown to be common among all phiKMV-related phages.

Original language	English
Pages (from-to)	1-4
Number of pages	4
Journal	Virology
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2009.06.024
Publication status	Published - 15 Aug 2009

ASJC Scopus subject areas

Virology

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10.1016/j.virol.2009.06.024

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title = "Genomes of {"}phiKMV-like viruses{"} of Pseudomonas aeruginosa contain localized single-strand interruptions",

abstract = "The {"}phiKMV-like viruses{"} comprise an important genus of T7 related phages infecting Pseudomonas aeruginosa. The genomes of these bacteriophages have localized single-strand interruptions (nicks), a distinguishing genomic trait previously thought to be unique for T5 related coliphages. Analysis of this feature in the newly sequenced phage fkF77 shows all four nicks to be localized on the non-coding DNA strand. They are present with high frequencies within the phage population and are introduced into the phage DNA at late stages of the lytic cycle. The general consensus sequence in the nicks (5'-CGACxxxxxCCTAoh pCTCCGG-3') was shown to be common among all phiKMV-related phages.",

author = "Leonid Kulakov and V.N. Ksenzenko and M.G. Shlyapnikov and V.V. Kochetkov and {Del Casale}, Antonio and Christopher Allen and Mike Larkin and P- Ceyssens and R. Lavigne",

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T1 - Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions

AU - Kulakov, Leonid

AU - Ksenzenko, V.N.

AU - Shlyapnikov, M.G.

AU - Kochetkov, V.V.

AU - Del Casale, Antonio

AU - Allen, Christopher

AU - Larkin, Mike

AU - Ceyssens, P-

AU - Lavigne, R.

PY - 2009/8/15

Y1 - 2009/8/15

N2 - The "phiKMV-like viruses" comprise an important genus of T7 related phages infecting Pseudomonas aeruginosa. The genomes of these bacteriophages have localized single-strand interruptions (nicks), a distinguishing genomic trait previously thought to be unique for T5 related coliphages. Analysis of this feature in the newly sequenced phage fkF77 shows all four nicks to be localized on the non-coding DNA strand. They are present with high frequencies within the phage population and are introduced into the phage DNA at late stages of the lytic cycle. The general consensus sequence in the nicks (5'-CGACxxxxxCCTAoh pCTCCGG-3') was shown to be common among all phiKMV-related phages.

AB - The "phiKMV-like viruses" comprise an important genus of T7 related phages infecting Pseudomonas aeruginosa. The genomes of these bacteriophages have localized single-strand interruptions (nicks), a distinguishing genomic trait previously thought to be unique for T5 related coliphages. Analysis of this feature in the newly sequenced phage fkF77 shows all four nicks to be localized on the non-coding DNA strand. They are present with high frequencies within the phage population and are introduced into the phage DNA at late stages of the lytic cycle. The general consensus sequence in the nicks (5'-CGACxxxxxCCTAoh pCTCCGG-3') was shown to be common among all phiKMV-related phages.

U2 - 10.1016/j.virol.2009.06.024

DO - 10.1016/j.virol.2009.06.024

M3 - Article

C2 - 19592061

SN - 0042-6822

VL - 391

SP - 1

EP - 4

JO - Virology

JF - Virology

IS - 1

ER -

Genomes of "phiKMV-like viruses" of Pseudomonas aeruginosa contain localized single-strand interruptions

Abstract

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