Genomic characterization of a large plasmid containing a bla NDM-1 gene carried on Salmonella enterica serovar Indiana C629 isolate from China

Wei Wang, Zulqarnain Baloch, Zixin Peng, Yujie Hu, Jin Xu, Séamus Fanning*, Fengqin Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)
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Abstract

Background: The bla NDM-1 gene in Salmonella species is mostly reported in clinical cases, but is rarely isolated from red and white meat in China. 

Methods: A Salmonella Indiana (S. Indiana) isolate was cultured from a chicken carcass procured from a slaughterhouse in China. Antimicrobial susceptibility was tested against a panel of agents. Whole-genome sequencing of the isolate was carried out and data was analyzed. 

Results: A large plasmid, denoted as plasmid pC629 (210,106 bp), containing a composite cassette, consisting of IS26-bla NDM-1-ble MBL -δtrpF-tat-cutA-ISCR1-sul1-qacEδ1-aadA2-dfrA12-intI1-IS26 was identified. The latter locus was physically linked with bla OXA-1, bla CTX-M-65, bla TEM-1-encoding genes. A mercury resistance operon merACDEPTR was also identified; it was flanked on the proximal side, among IS26 element and the distally located on the bla NDM-1 gene. Plasmid pC629 also contained 21 other antimicrobial resistance-encoding genes, such as aac(6')-Ib-cr, aac(3)-VI, aadA5, aph(4)-Ia, arr-3, blmS, brp, catB3, dfrA17, floR, fosA, mph(A), mphR, mrx, nimC/nimA, oqxA, oqxB, oqxR, rmtB, sul1, sul2. Two virulence genes were also identified on plasmid pC629. 

Conclusion: To the best of our knowledge, this is the first report of bla NDM-1 gene being identified from a plasmid in a S. Indiana isolate cultured from chicken carcass in China.

Original languageEnglish
Article number479
JournalBMC Infectious Diseases
Volume17
DOIs
Publication statusPublished - 07 Jul 2017

Bibliographical note

Funding Information:
We sincerely the National Key Research and Development Program of China, the Ministry of Science and Technology of the People’s Republic of China for funding. We also thank to Dr. Syed Jabir Hussain of Guangzhou Institute of Geochemistry, Chinese Academy of Science for assistance in manuscript revision.

Funding Information:
This study was supported by the National Key Research and Development Program of China, the Ministry of Science and Technology of the People’s Republic of China (2016YFD0401102) and China Food Safety Talent Competency Development Initiative: CFSA 523 Program. The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Publisher Copyright:
© 2017 The Author(s).

Keywords

  • bla
  • Carbapenem resistance
  • Chicken carcass
  • China
  • Extensively-drug resistance
  • gene
  • Salmonella enterica serovar Indiana

ASJC Scopus subject areas

  • Infectious Diseases

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