Genomovar diversity amongst Burkholderia cepacia complex isolates from an Australian adult cystic fibrosis unit

T J Kidd, S C Bell, C Coulter

Research output: Contribution to journalArticlepeer-review

19 Citations (Scopus)

Abstract

In this study, a combination of recA-based PCR assays and 16S rDNA restriction fragment length polymorphism (RFLP) analysis was used to determine the genomovar diversity of clinical Burkholderia cepacia complex isolates. Twenty-eight isolates were prospectively collected from patients attending a large Australian adult cystic fibrosis (CF) unit, 22 isolates were referred from other Australian CF units and a further eight isolates originated from patients without CF. The 28 prospectively collected isolates were distributed amongst the following genomovars: Burkholderia cepacia genomovar I (28.6%), Burkholderia multivorans (21.4%), Burkholderia cepacia genomovar III (39.3%), Burkholderia vietnamiensis(3.6%) and Burkholderia ambifaria (7.1%). The results of this study highlight the usefulness of 16S rDNA RFLP typing for the identification of other Burkholderia spp. and non-fermenting gram-negative bacteria.

Original languageEnglish
Pages (from-to)434-7
Number of pages4
JournalEuropean Journal of Clinical Microbiology & Infectious Diseases
Volume22
Issue number7
DOIs
Publication statusPublished - Jul 2003

Keywords

  • Adult
  • Animals
  • Australia
  • Burkholderia Infections
  • Burkholderia cepacia
  • Cystic Fibrosis
  • DNA, Bacterial
  • Genetic Variation
  • Humans
  • Polymerase Chain Reaction
  • Prospective Studies
  • Respiratory Tract Infections

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