Getting Down to Specifics: Profiling Gene Expression and Protein-DNA Interactions in a Cell Type-Specific Manner

Colin D McClure, Tony D Southall

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

The majority of multicellular organisms are comprised of an extraordinary range of cell types, with different properties and gene expression profiles. Understanding what makes each cell type unique and how their individual characteristics are attributed are key questions for both developmental and neurobiologists alike. The brain is an excellent example of the cellular diversity expressed in the majority of eukaryotes. The mouse brain comprises of approximately 75 million neurons varying in morphology, electrophysiology, and preferences for synaptic partners. A powerful process in beginning to pick apart the mechanisms that specify individual characteristics of the cell, as well as their fate, is to profile gene expression patterns, chromatin states, and transcriptional networks in a cell type-specific manner, i.e., only profiling the cells of interest in a particular tissue. Depending on the organism, the questions being investigated, and the material available, certain cell type-specific profiling methods are more suitable than others. This chapter reviews the approaches presently available for selecting and isolating specific cell types and evaluates their key features.

Original languageEnglish
Pages (from-to)103-151
Number of pages49
JournalCurrent Genetics
Volume91
DOIs
Publication statusPublished - 2015
Externally publishedYes

Fingerprint

DNA
Proteins
Transcriptome
Gene Regulatory Networks
Electrophysiology
Brain
Eukaryota
Chromatin
Neurons

Bibliographical note

Copyright © 2015 Elsevier Inc. All rights reserved.

Keywords

  • Animals
  • Cytological Techniques
  • DNA-Binding Proteins/metabolism
  • Gene Regulatory Networks
  • Humans
  • Organ Specificity
  • Transcriptome

Cite this

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Getting Down to Specifics: Profiling Gene Expression and Protein-DNA Interactions in a Cell Type-Specific Manner. / McClure, Colin D; Southall, Tony D.

In: Current Genetics, Vol. 91, 2015, p. 103-151.

Research output: Contribution to journalReview article

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AU - Southall, Tony D

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AB - The majority of multicellular organisms are comprised of an extraordinary range of cell types, with different properties and gene expression profiles. Understanding what makes each cell type unique and how their individual characteristics are attributed are key questions for both developmental and neurobiologists alike. The brain is an excellent example of the cellular diversity expressed in the majority of eukaryotes. The mouse brain comprises of approximately 75 million neurons varying in morphology, electrophysiology, and preferences for synaptic partners. A powerful process in beginning to pick apart the mechanisms that specify individual characteristics of the cell, as well as their fate, is to profile gene expression patterns, chromatin states, and transcriptional networks in a cell type-specific manner, i.e., only profiling the cells of interest in a particular tissue. Depending on the organism, the questions being investigated, and the material available, certain cell type-specific profiling methods are more suitable than others. This chapter reviews the approaches presently available for selecting and isolating specific cell types and evaluates their key features.

KW - Animals

KW - Cytological Techniques

KW - DNA-Binding Proteins/metabolism

KW - Gene Regulatory Networks

KW - Humans

KW - Organ Specificity

KW - Transcriptome

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