Getting to the heart of cardiac remodeling; how collagen subtypes may contribute to phenotype

P. Collier; C. J. Watson; M. H. van Es; D. Phelan; C. McGorrian; M. Tolan; M. T. Ledwidge; K. M. McDonald; J. A. Baugh

doi:10.1016/j.yjmcc.2011.10.002

Getting to the heart of cardiac remodeling; how collagen subtypes may contribute to phenotype

P. Collier, C. J. Watson, M. H. van Es, D. Phelan, C. McGorrian, M. Tolan, M. T. Ledwidge, K. M. McDonald, J. A. Baugh^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

The objective of this study was to investigate the nature and biomechanical properties of collagen fibers within the human myocardium. Targeting cardiac interstitial abnormalities will likely become a major focus of future preventative strategies with regard to the management of cardiac dysfunction. Current knowledge regarding the component structures of myocardial collagen networks is limited, further delineation of which will require application of more innovative technologies. We applied a novel methodology involving combined confocal laser scanning and atomic force microscopy to investigate myocardial collagen within ex-vivo right atrial tissue from 10 patients undergoing elective coronary bypass surgery. Immuno-fluorescent co-staining revealed discrete collagen I and III fibers. During single fiber deformation, overall median values of stiffness recorded in collagen III were 37±16% lower than in collagen I [p

Original language	English
Pages (from-to)	148-53
Number of pages	6
Journal	Journal of Molecular and Cellular Cardiology
Volume	52
Issue number	1
Early online date	10 Oct 2011
DOIs	https://doi.org/10.1016/j.yjmcc.2011.10.002
Publication status	Published - 02 Jan 2012
Externally published	Yes

Keywords

Aged
Atrial Fibrillation
Collagen Type I
Collagen Type III
Female
Humans
Male
Microscopy, Atomic Force
Middle Aged
Phenotype
Ventricular Remodeling

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Cite this

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abstract = "The objective of this study was to investigate the nature and biomechanical properties of collagen fibers within the human myocardium. Targeting cardiac interstitial abnormalities will likely become a major focus of future preventative strategies with regard to the management of cardiac dysfunction. Current knowledge regarding the component structures of myocardial collagen networks is limited, further delineation of which will require application of more innovative technologies. We applied a novel methodology involving combined confocal laser scanning and atomic force microscopy to investigate myocardial collagen within ex-vivo right atrial tissue from 10 patients undergoing elective coronary bypass surgery. Immuno-fluorescent co-staining revealed discrete collagen I and III fibers. During single fiber deformation, overall median values of stiffness recorded in collagen III were 37±16% lower than in collagen I [p",

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AU - Collier, P.

AU - Watson, C. J.

AU - van Es, M. H.

AU - Phelan, D.

AU - McGorrian, C.

AU - Tolan, M.

AU - Ledwidge, M. T.

AU - McDonald, K. M.

AU - Baugh, J. A.

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KW - Aged

KW - Atrial Fibrillation

KW - Collagen Type I

KW - Collagen Type III

KW - Female

KW - Humans

KW - Male

KW - Microscopy, Atomic Force

KW - Middle Aged

KW - Phenotype

KW - Ventricular Remodeling

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JO - Journal of Molecular and Cellular Cardiology

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Getting to the heart of cardiac remodeling; how collagen subtypes may contribute to phenotype

Abstract

Keywords

UN SDGs

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