Gingival fibroblast response to cyclosporin A and transforming growth factor β1

J. A. James*, C. R. Irwin, G. J. Linden

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


This study investigates a potential role for TGFβ1 in the pathogenesis of cyclosporin A-induced gingival overgrowth (CsA-OG). TGFβ1 was localized immunohistochemically in the connective tissue of both normal gingiva and CsA-OG. Intense staining for TGFβ1 was detected at the tips of the dermal papillae of the overgrown gingiva. In addition, fibroblasts derived from healthy gingiva and fibroblasts derived from CsA-OG were cultured both as monolayers or embedded in a 3D-collagen gel. Fibroblast activity was monitored in terms of protein and collagen production in the presence of (i) 1 ng/ml TGFβ1, (ii) 500 ng/ml CsA, or (iii) 500 ng/ml CsA and 1 ng/ml TGF/β1. In monolayer culture TGFβ1 significantly increased protein and collagen production in all cell strains (p<0.05); however, there was no difference in response between fibroblasts from overgrown and healthy tissue. The production of both protein and collagen was significantly lower in the presence of the combination of CsA and TGFβ1 when compared with the maximal stimulation produced by TGFβ1 alone. In gel, TGFβ1 significantly elevated matrix production by all overgrown cell strains (p<0.05) but had little or no effect on the normal cell strains. The combination of CsA and TGFβ1 in gel cultures reduced protein and collagen production by overgrown cell strains compared with TGFβ1 alone. It is concluded that the cellular activity of gingival fibroblasts is dependant on culture conditions and that fibroblasts derived from overgrown gingival tissue are more responsive to TGFβ1 than normal gingival fibroblasts when cultured in type I collagen gel.

Original languageEnglish
Pages (from-to)40-48
Number of pages9
JournalJournal of periodontal research
Issue number1
Publication statusPublished - 01 Jan 1998


  • Cyclosporin A
  • Fibroblasts
  • Gingival overgrowth
  • Transforming growth factor β

ASJC Scopus subject areas

  • Periodontics

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