Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions

Suneil Jain; Jonathan A. Coulter; Karl T. Butterworth; Alan R. Hounsell; Stephen J. McMahon; Wendy B. Hyland; Mark F. Muir; Glenn R. Dickson; Kevin M. Prise; Fred J. Currell; David G. Hirst; Joe M. O'Sullivan

doi:10.1016/j.radonc.2013.12.013

Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions

Suneil Jain^*, Jonathan A. Coulter, Karl T. Butterworth, Alan R. Hounsell, Stephen J. McMahon, Wendy B. Hyland, Mark F. Muir, Glenn R. Dickson, Kevin M. Prise, Fred J. Currell, David G. Hirst, Joe M. O'Sullivan

^*Corresponding author for this work

Research output: Contribution to journal › Article

47 Citations (Scopus)

Abstract

Background and purpose: Gold nanoparticles (GNPs) are novel agents that have been shown to cause radiosensitisation in vitro and in vivo. Tumour hypoxia is associated with radiation resistance and reduced survival in cancer patients. The interaction of GNPs with cells in hypoxia is explored.

Materials and methods: GNP uptake, localization, toxicity and radiosensitisation were assessed in vitro under oxic and hypoxic conditions.

Results: GNP cellular uptake was significantly lower under hypoxic than oxic conditions. A significant reduction in cell proliferation in hypoxic MDA-MB-231 breast cancer cells exposed to GNPs was observed. In these cells significant radiosensitisation occurred in normoxia and moderate hypoxia. However, in near anoxia no significant sensitisation occurred.

Conclusions: GNP uptake occurred in hypoxic conditions, causing radiosensitisation in moderate, but not extreme hypoxia in a breast cancer cell line. These findings may be important for the development of GNPs for cancer therapy.

Original language	English
Pages (from-to)	342-347
Number of pages	6
Journal	Radiotherapy and Oncology
Volume	110
Issue number	2
Early online date	17 Jan 2014
DOIs	https://doi.org/10.1016/j.radonc.2013.12.013
Publication status	Published - Feb 2014

Bibliographical note

Keywords

Gold nanoparticles
Radiosensitisers
Toxicity
Hypoxia
RADIATION-THERAPY
TUMOR HYPOXIA
CANCER-CELLS
IN-VIVO
LOCALIZATION
APOPTOSIS

Cite this

Jain, S., Coulter, J. A., Butterworth, K. T., Hounsell, A. R., McMahon, S. J., Hyland, W. B., Muir, M. F., Dickson, G. R., Prise, K. M., Currell, F. J., Hirst, D. G., & O'Sullivan, J. M. (2014). Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions. Radiotherapy and Oncology, 110(2), 342-347. https://doi.org/10.1016/j.radonc.2013.12.013

Jain, Suneil ; Coulter, Jonathan A. ; Butterworth, Karl T. ; Hounsell, Alan R. ; McMahon, Stephen J. ; Hyland, Wendy B. ; Muir, Mark F. ; Dickson, Glenn R. ; Prise, Kevin M. ; Currell, Fred J. ; Hirst, David G. ; O'Sullivan, Joe M. / Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions. In: Radiotherapy and Oncology. 2014 ; Vol. 110, No. 2. pp. 342-347.

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abstract = "Background and purpose: Gold nanoparticles (GNPs) are novel agents that have been shown to cause radiosensitisation in vitro and in vivo. Tumour hypoxia is associated with radiation resistance and reduced survival in cancer patients. The interaction of GNPs with cells in hypoxia is explored.Materials and methods: GNP uptake, localization, toxicity and radiosensitisation were assessed in vitro under oxic and hypoxic conditions.Results: GNP cellular uptake was significantly lower under hypoxic than oxic conditions. A significant reduction in cell proliferation in hypoxic MDA-MB-231 breast cancer cells exposed to GNPs was observed. In these cells significant radiosensitisation occurred in normoxia and moderate hypoxia. However, in near anoxia no significant sensitisation occurred.Conclusions: GNP uptake occurred in hypoxic conditions, causing radiosensitisation in moderate, but not extreme hypoxia in a breast cancer cell line. These findings may be important for the development of GNPs for cancer therapy.",

keywords = "Gold nanoparticles, Radiosensitisers, Toxicity, Hypoxia, RADIATION-THERAPY, TUMOR HYPOXIA, CANCER-CELLS, IN-VIVO, LOCALIZATION, APOPTOSIS",

author = "Suneil Jain and Coulter, {Jonathan A.} and Butterworth, {Karl T.} and Hounsell, {Alan R.} and McMahon, {Stephen J.} and Hyland, {Wendy B.} and Muir, {Mark F.} and Dickson, {Glenn R.} and Prise, {Kevin M.} and Currell, {Fred J.} and Hirst, {David G.} and O'Sullivan, {Joe M.}",

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Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions. / Jain, Suneil ; Coulter, Jonathan A.; Butterworth, Karl T.; Hounsell, Alan R.; McMahon, Stephen J.; Hyland, Wendy B.; Muir, Mark F.; Dickson, Glenn R.; Prise, Kevin M.; Currell, Fred J.; Hirst, David G.; O'Sullivan, Joe M.

In: Radiotherapy and Oncology, Vol. 110, No. 2, 02.2014, p. 342-347.

Research output: Contribution to journal › Article

TY - JOUR

T1 - Gold nanoparticle cellular uptake, toxicity and radiosensitisation in hypoxic conditions

AU - Jain, Suneil

AU - Coulter, Jonathan A.

AU - Butterworth, Karl T.

AU - Hounsell, Alan R.

AU - McMahon, Stephen J.

AU - Hyland, Wendy B.

AU - Muir, Mark F.

AU - Dickson, Glenn R.

AU - Prise, Kevin M.

AU - Currell, Fred J.

AU - Hirst, David G.

AU - O'Sullivan, Joe M.

PY - 2014/2

Y1 - 2014/2

N2 - Background and purpose: Gold nanoparticles (GNPs) are novel agents that have been shown to cause radiosensitisation in vitro and in vivo. Tumour hypoxia is associated with radiation resistance and reduced survival in cancer patients. The interaction of GNPs with cells in hypoxia is explored.Materials and methods: GNP uptake, localization, toxicity and radiosensitisation were assessed in vitro under oxic and hypoxic conditions.Results: GNP cellular uptake was significantly lower under hypoxic than oxic conditions. A significant reduction in cell proliferation in hypoxic MDA-MB-231 breast cancer cells exposed to GNPs was observed. In these cells significant radiosensitisation occurred in normoxia and moderate hypoxia. However, in near anoxia no significant sensitisation occurred.Conclusions: GNP uptake occurred in hypoxic conditions, causing radiosensitisation in moderate, but not extreme hypoxia in a breast cancer cell line. These findings may be important for the development of GNPs for cancer therapy.

AB - Background and purpose: Gold nanoparticles (GNPs) are novel agents that have been shown to cause radiosensitisation in vitro and in vivo. Tumour hypoxia is associated with radiation resistance and reduced survival in cancer patients. The interaction of GNPs with cells in hypoxia is explored.Materials and methods: GNP uptake, localization, toxicity and radiosensitisation were assessed in vitro under oxic and hypoxic conditions.Results: GNP cellular uptake was significantly lower under hypoxic than oxic conditions. A significant reduction in cell proliferation in hypoxic MDA-MB-231 breast cancer cells exposed to GNPs was observed. In these cells significant radiosensitisation occurred in normoxia and moderate hypoxia. However, in near anoxia no significant sensitisation occurred.Conclusions: GNP uptake occurred in hypoxic conditions, causing radiosensitisation in moderate, but not extreme hypoxia in a breast cancer cell line. These findings may be important for the development of GNPs for cancer therapy.

KW - Gold nanoparticles

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KW - IN-VIVO

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JO - Radiotherapy and Oncology

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SN - 0167-8140

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Access to Document

10.1016/j.radonc.2013.12.013