Green synthesis, characterization and antiepileptic activity of herbal nanoparticles of mimusops elengi in mice

The aim of the study was to green synthesis and evaluate the antiepileptic activity of herbal nanoparticles of Mimusops elengi (ME) on electrochemical convulsion models in mice. ME herbal nanoparticles (MEHNPs) were synthesized and characterized. The particles size and zeta potential of MEHNPs were found to be 24 nm and 29.5 mV respectively. FTIR study revealed that minor shifts of peaks may be due to capping, reduction and stabilization of MEHNPs. Electro convulsions (ECs) were induced using electro-convulsiometer and ear-clip electrodes with alternating current of 45 mA for 0.2 s stimulation. PTZ was administered intraperitoneally to mice at a dose of 105 mg/kg, which was the CD97 (97% convulsive dose for the clonic phase) for inducing chemo-convulsions. The ability of methanol extract of ME bark (MEME-B) and ME herbal nanoparticles (MEHNPs) to reduce the hind limb extension (HLE) in EC and clonic-type (CT) convulsions in the PTZ model was used to establish antiepileptic parameters. HLE and CC was reduced significantly (P < 0.05) whereas neurochemical analyses show a significant rise in Acetylcholinesterase (AChE) and Gamma Butyric acid (GABA) levels. Antioxidant studies revealed that both MEME and MEHNPs significantly increased antioxidant enzymes and scavenged free radicals (P < 0.05), whereas histological findings revealed the protective effects. In both models, MEME-B and MEHNPs exhibited significant anti-epileptic properties, which could be attributed to suppressing excitatory neurohumoral transmission and enhanced inhibitory neurotransmission, as well as a significant increase in antioxidant status. MEHNPs, on the other hand, have outperformed MEME-B in terms of reducing oxidative stress and altering neurochemicals such as AChE and GABA.