Guidelines and considerations for conducting experiments using tissue microarrays

Mohammad Ilyas, Heike Grabsch, Ian O Ellis, Chris Womack, Robert Brown, Dan Berney, Dean Fennell, Manuel Salto-Tellez, Martin Jenkins, Goran Landberg, Richard Byers, Darren Treanor, David Harrison, Andrew R Green, Graham Ball, Peter Hamilton

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Tissue microarrays (TMAs) represent a powerful method for undertaking large-scale tissue-based biomarker studies. While TMAs offer several advantages, there are a number of issues specific to their use which need to be considered when employing this method. Given the investment in TMA-based research, guidance on design and execution of experiments will be of benefit and should help researchers new to TMA-based studies to avoid known pitfalls. Furthermore, a consensus on quality standards for TMA-based experiments should improve the robustness and reproducibility of studies, thereby increasing the likelihood of identifying clinically useful biomarkers. In order to address these issues, the National Cancer Research Institute Biomarker and Imaging Clinical Studies Group organized a 1-day TMA workshop held in Nottingham in May 2012. The document herein summarizes the conclusions from the workshop. It includes guidance and considerations on all aspects of TMA-based research, including the pre-analytical stages of experimental design, the analytical stages of data acquisition, and the postanalytical stages of data analysis. A checklist is presented which can be used both for planning a TMA experiment and interpreting the results of such an experiment. For studies of cancer biomarkers, this checklist could be used as a supplement to the REMARK guidelines.
Original languageEnglish
Pages (from-to)827-839
JournalHistopathology
Volume62
Issue number6
Early online date12 Apr 2013
DOIs
Publication statusPublished - May 2013

Bibliographical note

© 2013 Blackwell Publishing Ltd.

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

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