Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia

Letizia Foroni; Gill Wilson; Gareth Gerrard; Joanne Mason; David Grimwade; Helen E White; David Gonzalez de Castro; Stephen Austin; Abida Awan; Emma Burt; Tim Clench; Joanna Farruggia; Jeremy Hancock; Alexandra E Irvine; Aytug Kizilors; Stephen Langabeer; Benedict J Milner; Guillermina Nickless; Anna Schuh; Anne Sproul; Lihui Wang; Caroline Wickham; Nicholas C P Cross

doi:10.1111/j.1365-2141.2011.08603.x

Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia

Letizia Foroni, Gill Wilson, Gareth Gerrard, Joanne Mason, David Grimwade, Helen E White, David Gonzalez de Castro, Stephen Austin, Abida Awan, Emma Burt, Tim Clench, Joanna Farruggia, Jeremy Hancock, Alexandra E Irvine, Aytug Kizilors, Stephen Langabeer, Benedict J Milner, Guillermina Nickless, Anna Schuh, Anne SproulLihui Wang, Caroline Wickham, Nicholas C P Cross

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

Molecular testing for the BCR-ABL1 fusion gene by real time quantitative polymerase chain reaction (RT-qPCR) is the most sensitive routine approach for monitoring the response to therapy of patients with chronic myeloid leukaemia. In the context of tyrosine kinase inhibitor (TKI) therapy, the technique is most appropriate for patients who have achieved complete cytogenetic remission and can be used to define specific therapeutic milestones. To achieve this effectively, standardization of the laboratory procedures and the interpretation of results are essential. We present here consensus best practice guidelines for RT-qPCR testing, data interpretation and reporting that have been drawn up and agreed by a consortium of 21 testing laboratories in the United Kingdom and Ireland in accordance with the procedures of the UK Clinical Molecular Genetics Society.

Original language	English
Pages (from-to)	179-90
Number of pages	12
Journal	British Journal of Haematology
Volume	153
Issue number	2
Early online date	08 Mar 2011
DOIs	https://doi.org/10.1111/j.1365-2141.2011.08603.x
Publication status	Published - Apr 2011

Bibliographical note

Keywords

Fusion Proteins, bcr-abl
Great Britain
Humans
Ireland
Leukemia, Myelogenous, Chronic, BCR-ABL Positive
Molecular Biology
Monitoring, Physiologic
Practice Guidelines as Topic
Protein Kinase Inhibitors
RNA, Messenger
RNA, Neoplasm
Reverse Transcriptase Polymerase Chain Reaction
Societies, Medical

ASJC Scopus subject areas

Hematology

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10.1111/j.1365-2141.2011.08603.x

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Foroni, L., Wilson, G., Gerrard, G., Mason, J., Grimwade, D., White, H. E., de Castro, D. G., Austin, S., Awan, A., Burt, E., Clench, T., Farruggia, J., Hancock, J., Irvine, A. E., Kizilors, A., Langabeer, S., Milner, B. J., Nickless, G., Schuh, A., ... Cross, N. C. P. (2011). Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia. British Journal of Haematology, 153(2), 179-90. https://doi.org/10.1111/j.1365-2141.2011.08603.x

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abstract = "Molecular testing for the BCR-ABL1 fusion gene by real time quantitative polymerase chain reaction (RT-qPCR) is the most sensitive routine approach for monitoring the response to therapy of patients with chronic myeloid leukaemia. In the context of tyrosine kinase inhibitor (TKI) therapy, the technique is most appropriate for patients who have achieved complete cytogenetic remission and can be used to define specific therapeutic milestones. To achieve this effectively, standardization of the laboratory procedures and the interpretation of results are essential. We present here consensus best practice guidelines for RT-qPCR testing, data interpretation and reporting that have been drawn up and agreed by a consortium of 21 testing laboratories in the United Kingdom and Ireland in accordance with the procedures of the UK Clinical Molecular Genetics Society.",

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Foroni, L, Wilson, G, Gerrard, G, Mason, J, Grimwade, D, White, HE, de Castro, DG, Austin, S, Awan, A, Burt, E, Clench, T, Farruggia, J, Hancock, J, Irvine, AE, Kizilors, A, Langabeer, S, Milner, BJ, Nickless, G, Schuh, A, Sproul, A, Wang, L, Wickham, C & Cross, NCP 2011, 'Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia', British Journal of Haematology, vol. 153, no. 2, pp. 179-90. https://doi.org/10.1111/j.1365-2141.2011.08603.x

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AU - Foroni, Letizia

AU - Wilson, Gill

AU - Gerrard, Gareth

AU - Mason, Joanne

AU - Grimwade, David

AU - White, Helen E

AU - de Castro, David Gonzalez

AU - Austin, Stephen

AU - Awan, Abida

AU - Burt, Emma

AU - Clench, Tim

AU - Farruggia, Joanna

AU - Hancock, Jeremy

AU - Irvine, Alexandra E

AU - Kizilors, Aytug

AU - Langabeer, Stephen

AU - Milner, Benedict J

AU - Nickless, Guillermina

AU - Schuh, Anna

AU - Sproul, Anne

AU - Wang, Lihui

AU - Wickham, Caroline

AU - Cross, Nicholas C P

PY - 2011/4

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N2 - Molecular testing for the BCR-ABL1 fusion gene by real time quantitative polymerase chain reaction (RT-qPCR) is the most sensitive routine approach for monitoring the response to therapy of patients with chronic myeloid leukaemia. In the context of tyrosine kinase inhibitor (TKI) therapy, the technique is most appropriate for patients who have achieved complete cytogenetic remission and can be used to define specific therapeutic milestones. To achieve this effectively, standardization of the laboratory procedures and the interpretation of results are essential. We present here consensus best practice guidelines for RT-qPCR testing, data interpretation and reporting that have been drawn up and agreed by a consortium of 21 testing laboratories in the United Kingdom and Ireland in accordance with the procedures of the UK Clinical Molecular Genetics Society.

AB - Molecular testing for the BCR-ABL1 fusion gene by real time quantitative polymerase chain reaction (RT-qPCR) is the most sensitive routine approach for monitoring the response to therapy of patients with chronic myeloid leukaemia. In the context of tyrosine kinase inhibitor (TKI) therapy, the technique is most appropriate for patients who have achieved complete cytogenetic remission and can be used to define specific therapeutic milestones. To achieve this effectively, standardization of the laboratory procedures and the interpretation of results are essential. We present here consensus best practice guidelines for RT-qPCR testing, data interpretation and reporting that have been drawn up and agreed by a consortium of 21 testing laboratories in the United Kingdom and Ireland in accordance with the procedures of the UK Clinical Molecular Genetics Society.

KW - Fusion Proteins, bcr-abl

KW - Great Britain

KW - Humans

KW - Ireland

KW - Leukemia, Myelogenous, Chronic, BCR-ABL Positive

KW - Molecular Biology

KW - Monitoring, Physiologic

KW - Practice Guidelines as Topic

KW - Protein Kinase Inhibitors

KW - RNA, Messenger

KW - RNA, Neoplasm

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Societies, Medical

U2 - 10.1111/j.1365-2141.2011.08603.x

DO - 10.1111/j.1365-2141.2011.08603.x

M3 - Article

C2 - 21382019

VL - 153

SP - 179

EP - 190

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 2

ER -

Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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