Hi-C as a tool for precise detection and characterisation of chromosomal rearrangements and copy number variation in human tumours.

Louise Harewood, Kamal Kishore, Matthew D Eldridge, Steven Wingett, Danita Pearson, Stefan Schoenfelder, V Peter Collins, Peter Fraser

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)
156 Downloads (Pure)

Abstract

Chromosomal rearrangements occur constitutionally in the general population and somatically in the majority of cancers. Detection of balanced rearrangements, such as reciprocal translocations and inversions, is troublesome, which is particularly detrimental in oncology where rearrangements play diagnostic and prognostic roles. Here we describe the use of Hi-C as a tool for detection of both balanced and unbalanced chromosomal rearrangements in primary human tumour samples, with the potential to define chromosome breakpoints to bp resolution. In addition, we show copy number profiles can also be obtained from the same data, all at a significantly lower cost than standard sequencing approaches.
Original languageEnglish
Article number125
Number of pages11
JournalGenome biology
Volume18
DOIs
Publication statusPublished - 27 Jun 2017

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