Hierachically structured hollow silica spheres for high efficiency immobilization of enzymes

Shunsheng Cao*, Long Fang, Zhiyuan Zhao, Yi Ge, Sergey Piletsky, Anthony P F Turner

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)


In this work, the first example of a hierarchically structured hollow silica system is reported without any chemical modification to the enzyme involved in the process. The leaching of the physically adsorbed enzyme is substantially restrained in comparison to pure hollow silica supports. The hierarchical architecture is composed of the ordered hollow silica spheres with a shell-in-shell structure. This rationally integrated architecture, which serves as the host for glucose oxidase immobilization, displays many significant advantages, including increases in mechanical stability, enzyme loading, and bioactivity, and a decrease in enzyme leaching compared to existing pure hollow silica matrices. This facilitates further multifarious applications for enhanced enzyme immobilization, biosensors, and biocatalysis.

Original languageEnglish
Pages (from-to)2162-2167
Number of pages6
JournalAdvanced Functional Materials
Issue number17
Early online date22 Nov 2012
Publication statusPublished - 06 May 2013
Externally publishedYes


  • enzyme immobilization
  • glucose oxidase
  • hierarchically structured particles
  • hollow silica

ASJC Scopus subject areas

  • Biomaterials
  • Electrochemistry
  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials

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