High-Frequency Ultrasound Assessment of the Murine Heart From Embryo Through to Juvenile

N. Corrigan, Derek Brazil, F.M. Auliffe

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Aim: The aim of this study is to assess the murine heart of normal embryos, neonates, and juveniles using high-frequency ultrasound. Methods: Diastolic function was measured with E/A ratio (E wave velocity/A wave velocity) and isovolumetric relaxation time (IRT), systolic function with isovolumetric contraction time (ICT), percentage fractional shortening (FS%), percentage ejection fraction (EF%). Global cardiac performance was quantified using myocardial performance index (MPI). Results: Isovolumetric relaxation time remained stable from E10.5 to 3 weeks. Systolic function (ICT) improved with gestation and remained stable from E18.5 onward. Myocardial performance index showed improvement in embryonic lift (0.82-0.63) and then stabilized from 1 to 3 week (0.60-0.58). Percentage ejection fraction remained high during gestation (77%-69%) and then decreased from the neonate to juvenile (68%-51%). Conclusion: The ultrasound biomicroscope allows for noninvasive in-depth assessment of cardiac function of embryos and pups. Detailed physiological and functional cardiac function readouts can be obtained, which is invaluable for comparison to mouse models of disease.
Original languageEnglish
Pages (from-to)147-157
Number of pages11
JournalReproductive sciences (Thousand Oaks, Calif.)
Volume17
Issue number2
DOIs
Publication statusPublished - Feb 2010

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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