High-performance liquid-chromatographic separation of ofloxacin using a chiral stationary phase

Xiaojie Sun; Di Wu; Bing Shao; Jing Zhang

doi:10.2116/analsci.25.931

High-performance liquid-chromatographic separation of ofloxacin using a chiral stationary phase

Xiaojie Sun
, Di Wu
, Bing Shao^*
, Jing Zhang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm × 4.6 mm, 5 μm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers. 2009

Original language	English
Pages (from-to)	931-933
Number of pages	3
Journal	Analytical Sciences
Volume	25
Issue number	7
DOIs	https://doi.org/10.2116/analsci.25.931
Publication status	Published - 10 Jul 2009
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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abstract = "A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm × 4.6 mm, 5 μm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers. 2009",

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AU - Sun, Xiaojie

AU - Wu, Di

AU - Shao, Bing

AU - Zhang, Jing

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AB - A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm × 4.6 mm, 5 μm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the mobile phase has played an important role in enhancing the chromatographic efficiency and resolution between enantiomers. 2009

U2 - 10.2116/analsci.25.931

DO - 10.2116/analsci.25.931

M3 - Article

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EP - 933

JO - Analytical Sciences

JF - Analytical Sciences

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ER -

High-performance liquid-chromatographic separation of ofloxacin using a chiral stationary phase

Abstract

ASJC Scopus subject areas

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