High-performance size-exclusion chromatography of peptides

Brent Irvine

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


Gel filtration on soft gels has been employed for over 40 years for the separation, desalting and molecular weight estimation of peptides and proteins. Technical improvements have given rise to high-performance size-exclusion chromatography (HPSEC) on rigid supports, giving more rapid run times and increased resolution. Initially, these packings were more suitable for the separation of proteins than of peptides, but supports that operate in the fractionation range
Original languageEnglish
Pages (from-to)233-242
Number of pages10
JournalJournal of Biochemical and Biophysical Methods
Issue number1-3
Publication statusPublished - 30 Jun 2003

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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