Abstract
Gel filtration on soft gels has been employed for over 40 years for the separation, desalting and molecular weight estimation of peptides and proteins. Technical improvements have given rise to high-performance size-exclusion chromatography (HPSEC) on rigid supports, giving more rapid run times and increased resolution. Initially, these packings were more suitable for the separation of proteins than of peptides, but supports that operate in the fractionation range
| Original language | English |
|---|---|
| Pages (from-to) | 233-242 |
| Number of pages | 10 |
| Journal | Journal of Biochemical and Biophysical Methods |
| Volume | 56 |
| Issue number | 1-3 |
| Publication status | Published - 30 Jun 2003 |
ASJC Scopus subject areas
- Biochemistry
- Biophysics