High resolution mass spectrometry based profiling of diet-related deoxyribonucleic acid adducts

L.Y. Hemeryck, A.I. Decloedt, J. Vanden Bussche, K.P. Geboes, L. Vanhaecke

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Exposure of DNA to endo- and exogenous DNA binding chemicals can result in the formation of DNA adducts and is believed to be the first step in chemically induced carcinogenesis. DNA adductomics is a relatively new field of research which studies the formation of known and unknown DNA adducts in DNA due to exposure to genotoxic chemicals. In this study, a new UHPLC-HRMS(/MS)-based DNA adduct detection method was developed and validated. Four targeted DNA adducts, which all have been linked to dietary genotoxicity, were included in the described method; O6-methylguanine (O6-MeG), O6-carboxymethylguanine (O6-CMG), pyrimidopurinone (M1G) and methylhydroxypropanoguanine (CroG). As a supplementary tool for DNA adductomics, a DNA adduct database, which currently contains 123 different diet-related DNA adducts, was constructed. By means of the newly developed method and database, all 4 targeted DNA adducts and 32 untargeted DNA adducts could be detected in different DNA samples. The obtained results clearly demonstrate the merit of the described method for both targeted and untargeted DNA adduct detection in vitro and in vivo, whilst the diet-related DNA adduct database can distinctly facilitate data interpretation. © 2015 Elsevier B.V.
Original languageEnglish
Pages (from-to)123-131
JournalAnalytica Chimica Acta
Volume892
Early online date22 Aug 2015
DOIs
Publication statusPublished - 10 Sep 2015

Bibliographical note

cited By 16

Keywords

  • Chemicals
  • Database systems
  • Diseases
  • DNA
  • Mass spectrometry
  • Organic acids
  • Spectrometry, Colorectal cancer
  • Data interpretation
  • Detection methods
  • DNA adducts
  • DNA binding
  • Genotoxicities
  • High resolution mass spectrometry
  • Orbitrap, Nucleic acids, 6 o methylguanine
  • chemical compound
  • guanine derivative
  • methylhydroxypropanoguanine
  • o 6 carboxymethylguanine
  • purine derivative
  • pyrimidopurinone
  • unclassified drug
  • calf thymus DNA
  • DNA adduct
  • guanine
  • O-(6)-methylguanine, adult
  • aged
  • Article
  • biomics
  • chemical carcinogenesis
  • clinical article
  • controlled study
  • data base
  • diet
  • DNA adductomics
  • female
  • genotoxicity
  • human
  • human tissue
  • in vitro study
  • in vivo study
  • male
  • mass spectrometry
  • priority journal
  • ultra performance liquid chromatography
  • analogs and derivatives
  • analysis
  • animal
  • bovine
  • chemical database
  • chemistry
  • colon tumor
  • high performance liquid chromatography
  • metabolism
  • middle aged
  • pathology
  • tandem mass spectrometry
  • very elderly, Aged
  • Aged, 80 and over
  • Animals
  • Cattle
  • Chromatography, High Pressure Liquid
  • Colonic Neoplasms
  • Databases, Chemical
  • Diet
  • DNA Adducts
  • Female
  • Guanine
  • Humans
  • Male
  • Middle Aged
  • Tandem Mass Spectrometry

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