High-throughput time-lapse fluorescence microscopy screening for heterogeneously expressed genes in bacillus subtilis

Julien Mortier; Stefanie Van Riet; Diana Senovilla Herrero; Kristof Vanoirbeek; Abram Aertsen

doi:10.1128/spectrum.02045-21

High-throughput time-lapse fluorescence microscopy screening for heterogeneously expressed genes in bacillus subtilis

Julien Mortier
, Stefanie Van Riet
, Diana Senovilla Herrero
, Kristof Vanoirbeek
, Abram Aertsen^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

74 Downloads (Pure)

Abstract

Elucidating phenotypic heterogeneity in clonal bacterial populations is important for both the fundamental understanding of bacterial behavior and the synthetic engineering of bacteria in biotechnology. In this study, we present and validate a high-throughput and high-resolution time-lapse fluorescence microscopy-based strategy to easily and systematically screen for heterogeneously expressed genes in the Bacillus subtilis model bacterium. This screen allows detection of expression patterns at high spatial and temporal resolution, which often escape detection by other approaches, and can readily be extrapolated to other bacteria. A proof-of-concept screening in B. subtilis revealed both recognized and yet unrecognized heterogeneously expressed genes, thereby validating the approach.

Original language	English
Article number	e02045-21
Journal	Microbiology Spectrum
Volume	10
Issue number	1
DOIs	https://doi.org/10.1128/spectrum.02045-21
Publication status	Published - 16 Feb 2022
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by a doctoral fellowship (11B0519N, to J.M.), a research grants (G0C7118N and G0B5616N) from the Research Foundation—Flanders (FWO-Vlaanderen), and a postdoctoral fellowship (PDM/20/118, to J.M.) from the KU Leuven Research Fund.

Publisher Copyright:
© 2022 Mortier et al.

Keywords

Bacillus subtilis
Gene expression
Population heterogeneity
Time-lapse fluorescence microscopy

ASJC Scopus subject areas

Physiology
Ecology
General Immunology and Microbiology
Genetics
Microbiology (medical)
Cell Biology
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/spectrum.02045-21Licence: CC BY

High-throughput time-lapse fluorescence microscopy screening for heterogeneously expressed genes in bacillus subtilis
Copyright 2022 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 1.97 MBLicence: CC BY

Cite this

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abstract = "Elucidating phenotypic heterogeneity in clonal bacterial populations is important for both the fundamental understanding of bacterial behavior and the synthetic engineering of bacteria in biotechnology. In this study, we present and validate a high-throughput and high-resolution time-lapse fluorescence microscopy-based strategy to easily and systematically screen for heterogeneously expressed genes in the Bacillus subtilis model bacterium. This screen allows detection of expression patterns at high spatial and temporal resolution, which often escape detection by other approaches, and can readily be extrapolated to other bacteria. A proof-of-concept screening in B. subtilis revealed both recognized and yet unrecognized heterogeneously expressed genes, thereby validating the approach.",

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AU - Mortier, Julien

AU - Van Riet, Stefanie

AU - Herrero, Diana Senovilla

AU - Vanoirbeek, Kristof

AU - Aertsen, Abram

N1 - Funding Information: This work was supported by a doctoral fellowship (11B0519N, to J.M.), a research grants (G0C7118N and G0B5616N) from the Research Foundation—Flanders (FWO-Vlaanderen), and a postdoctoral fellowship (PDM/20/118, to J.M.) from the KU Leuven Research Fund. Publisher Copyright: © 2022 Mortier et al.

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N2 - Elucidating phenotypic heterogeneity in clonal bacterial populations is important for both the fundamental understanding of bacterial behavior and the synthetic engineering of bacteria in biotechnology. In this study, we present and validate a high-throughput and high-resolution time-lapse fluorescence microscopy-based strategy to easily and systematically screen for heterogeneously expressed genes in the Bacillus subtilis model bacterium. This screen allows detection of expression patterns at high spatial and temporal resolution, which often escape detection by other approaches, and can readily be extrapolated to other bacteria. A proof-of-concept screening in B. subtilis revealed both recognized and yet unrecognized heterogeneously expressed genes, thereby validating the approach.

AB - Elucidating phenotypic heterogeneity in clonal bacterial populations is important for both the fundamental understanding of bacterial behavior and the synthetic engineering of bacteria in biotechnology. In this study, we present and validate a high-throughput and high-resolution time-lapse fluorescence microscopy-based strategy to easily and systematically screen for heterogeneously expressed genes in the Bacillus subtilis model bacterium. This screen allows detection of expression patterns at high spatial and temporal resolution, which often escape detection by other approaches, and can readily be extrapolated to other bacteria. A proof-of-concept screening in B. subtilis revealed both recognized and yet unrecognized heterogeneously expressed genes, thereby validating the approach.

KW - Bacillus subtilis

KW - Gene expression

KW - Population heterogeneity

KW - Time-lapse fluorescence microscopy

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ER -

High-throughput time-lapse fluorescence microscopy screening for heterogeneously expressed genes in bacillus subtilis

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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Cite this