Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates

Rashmi Mahajan, Mona Rouhi, Sudhirkumar Shinde, Thomas Bedwell, Anil Incel, Liliia Mavliutova, Sergey Piletsky, Ian A. Nicholls, Börje Sellergren*

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Citations (Scopus)
100 Downloads (Pure)

Abstract

We report an approach integrating the synthesis of protein-imprinted nanogels (“plastic antibodies”) with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino-functionalized solgel-coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high-dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein-conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited K d <10 pM for their respective target protein and low cross-reactivity with five reference proteins. This agrees with affinities reported for solid-phase-synthesized nanogels prepared using low-surface-area glass-bead supports. This approach simplifies the development and production of plastic antibodies and offers direct access to a practical bioassay.

Original languageEnglish
Pages (from-to)727-730
Number of pages4
JournalAngewandte Chemie - International Edition
Volume58
Issue number3
Early online date15 Nov 2018
DOIs
Publication statusPublished - 14 Jan 2019

Keywords

  • competitive assays
  • molecularly imprinted polymers
  • nanogel synthesis
  • plastic antibodies

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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