Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates

Rashmi Mahajan, Mona Rouhi, Sudhirkumar Shinde, Thomas Bedwell, Anil Incel, Liliia Mavliutova, Sergey Piletsky, Ian A. Nicholls, Börje Sellergren*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Citations (Scopus)
40 Downloads (Pure)

Abstract

We report an approach integrating the synthesis of protein-imprinted nanogels (“plastic antibodies”) with a highly sensitive assay employing templates attached to magnetic carriers. The enzymes trypsin and pepsin were immobilized on amino-functionalized solgel-coated magnetic nanoparticles (magNPs). Lightly crosslinked fluorescently doped polyacrylamide nanogels were subsequently produced by high-dilution polymerization of monomers in the presence of the magNPs. The nanogels were characterised by a novel competitive fluorescence assay employing identical protein-conjugated nanoparticles as ligands to reversibly immobilize the corresponding nanogels. Both nanogels exhibited K d <10 pM for their respective target protein and low cross-reactivity with five reference proteins. This agrees with affinities reported for solid-phase-synthesized nanogels prepared using low-surface-area glass-bead supports. This approach simplifies the development and production of plastic antibodies and offers direct access to a practical bioassay.

Original languageEnglish
Pages (from-to)727-730
Number of pages4
JournalAngewandte Chemie - International Edition
Volume58
Issue number3
Early online date15 Nov 2018
DOIs
Publication statusPublished - 14 Jan 2019

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Assays
Proteins
Nanoparticles
Antibodies
Plastics
Bioassay
Polyacrylates
Dilution
Enzymes
Monomers
Pepsin A
Fluorescence
Ligands
Polymerization
Glass
Trypsin
NanoGel

Keywords

  • competitive assays
  • molecularly imprinted polymers
  • nanogel synthesis
  • plastic antibodies

Cite this

Mahajan, Rashmi ; Rouhi, Mona ; Shinde, Sudhirkumar ; Bedwell, Thomas ; Incel, Anil ; Mavliutova, Liliia ; Piletsky, Sergey ; Nicholls, Ian A. ; Sellergren, Börje. / Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates. In: Angewandte Chemie - International Edition. 2019 ; Vol. 58, No. 3. pp. 727-730.
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Mahajan, R, Rouhi, M, Shinde, S, Bedwell, T, Incel, A, Mavliutova, L, Piletsky, S, Nicholls, IA & Sellergren, B 2019, 'Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates', Angewandte Chemie - International Edition, vol. 58, no. 3, pp. 727-730. https://doi.org/10.1002/anie.201805772

Highly Efficient Synthesis and Assay of Protein-Imprinted Nanogels by Using Magnetic Templates. / Mahajan, Rashmi; Rouhi, Mona; Shinde, Sudhirkumar; Bedwell, Thomas; Incel, Anil; Mavliutova, Liliia; Piletsky, Sergey; Nicholls, Ian A.; Sellergren, Börje.

In: Angewandte Chemie - International Edition, Vol. 58, No. 3, 14.01.2019, p. 727-730.

Research output: Contribution to journalArticle

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