Human B cell colony growth from pre-B cells in vitro

A C Fay, A Trudgett, Jonathan Thompson, T A McNeill

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

We describe a simple one-step technique for the growth of human B cell colonies in semi-solid agar in vitro. This method used conditioned medium from the human plasmacytoma cell line LICR-LON-H My 2 as a source of stimulating activity. A linear relationship exists between the number of B cells seeded and the number of colonies formed (r = 0.95). Most colony forming cells, approximately 1 in 500 of B cells seeded, lack surface immunoglobulin, possess Fc receptors and mark with the Leu 12 monoclonal antibody. Cells within developing colonies are found to have cytoplasmic IgM, IgA and IgG depending on the length of time in culture.
Original languageEnglish
Pages (from-to)629-36
Number of pages8
JournalClinical and experimental immunology
Volume59
Issue number3
Publication statusPublished - Mar 1985

Keywords

  • B-Lymphocytes
  • Cell Division
  • Cell Line
  • Cells, Cultured
  • Culture Media
  • Humans
  • Leukocyte Count
  • Methods
  • Plasmacytoma
  • Stem Cells

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