Human B cell colony growth from pre-B cells in vitro

A C Fay; A Trudgett; Jonathan Thompson; T A McNeill

Human B cell colony growth from pre-B cells in vitro

A C Fay, A Trudgett, Jonathan Thompson, T A McNeill

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We describe a simple one-step technique for the growth of human B cell colonies in semi-solid agar in vitro. This method used conditioned medium from the human plasmacytoma cell line LICR-LON-H My 2 as a source of stimulating activity. A linear relationship exists between the number of B cells seeded and the number of colonies formed (r = 0.95). Most colony forming cells, approximately 1 in 500 of B cells seeded, lack surface immunoglobulin, possess Fc receptors and mark with the Leu 12 monoclonal antibody. Cells within developing colonies are found to have cytoplasmic IgM, IgA and IgG depending on the length of time in culture.

Original language	English
Pages (from-to)	629-36
Number of pages	8
Journal	Clinical and Experimental Immunology
Volume	59
Issue number	3
Publication status	Published - Mar 1985

Keywords

B-Lymphocytes
Cell Division
Cell Line
Cells, Cultured
Culture Media
Humans
Leukocyte Count
Methods
Plasmacytoma
Stem Cells

Cite this

@article{25703fa9e39f43c2905084c15d51f1cb,

title = "Human B cell colony growth from pre-B cells in vitro",

abstract = "We describe a simple one-step technique for the growth of human B cell colonies in semi-solid agar in vitro. This method used conditioned medium from the human plasmacytoma cell line LICR-LON-H My 2 as a source of stimulating activity. A linear relationship exists between the number of B cells seeded and the number of colonies formed (r = 0.95). Most colony forming cells, approximately 1 in 500 of B cells seeded, lack surface immunoglobulin, possess Fc receptors and mark with the Leu 12 monoclonal antibody. Cells within developing colonies are found to have cytoplasmic IgM, IgA and IgG depending on the length of time in culture.",

keywords = "B-Lymphocytes, Cell Division, Cell Line, Cells, Cultured, Culture Media, Humans, Leukocyte Count, Methods, Plasmacytoma, Stem Cells",

author = "Fay, {A C} and A Trudgett and Jonathan Thompson and McNeill, {T A}",

year = "1985",

month = mar,

language = "English",

volume = "59",

pages = "629--36",

journal = "Clinical and Experimental Immunology",

issn = "0009-9104",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Human B cell colony growth from pre-B cells in vitro

AU - Fay, A C

AU - Trudgett, A

AU - Thompson, Jonathan

AU - McNeill, T A

PY - 1985/3

Y1 - 1985/3

N2 - We describe a simple one-step technique for the growth of human B cell colonies in semi-solid agar in vitro. This method used conditioned medium from the human plasmacytoma cell line LICR-LON-H My 2 as a source of stimulating activity. A linear relationship exists between the number of B cells seeded and the number of colonies formed (r = 0.95). Most colony forming cells, approximately 1 in 500 of B cells seeded, lack surface immunoglobulin, possess Fc receptors and mark with the Leu 12 monoclonal antibody. Cells within developing colonies are found to have cytoplasmic IgM, IgA and IgG depending on the length of time in culture.

AB - We describe a simple one-step technique for the growth of human B cell colonies in semi-solid agar in vitro. This method used conditioned medium from the human plasmacytoma cell line LICR-LON-H My 2 as a source of stimulating activity. A linear relationship exists between the number of B cells seeded and the number of colonies formed (r = 0.95). Most colony forming cells, approximately 1 in 500 of B cells seeded, lack surface immunoglobulin, possess Fc receptors and mark with the Leu 12 monoclonal antibody. Cells within developing colonies are found to have cytoplasmic IgM, IgA and IgG depending on the length of time in culture.

KW - B-Lymphocytes

KW - Cell Division

KW - Cell Line

KW - Cells, Cultured

KW - Culture Media

KW - Humans

KW - Leukocyte Count

KW - Methods

KW - Plasmacytoma

KW - Stem Cells

M3 - Article

C2 - 3872752

SN - 0009-9104

VL - 59

SP - 629

EP - 636

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

IS - 3

ER -

Human B cell colony growth from pre-B cells in vitro

Abstract

Keywords

Fingerprint

Cite this