TY - JOUR
T1 - Human cytochrome P450s are pro-oxidants in iron/ascorbate-initiated microsomal lipid peroxidation
AU - Lambert, Nigel
AU - Chambers, Stephen J.
AU - Plumb, Geoffrey W.
AU - Williamson, Gary
PY - 1996
Y1 - 1996
N2 - We have examined the effect of human cytochrome P450's (1A1,1A2,3A4,2A6,2B6,2D6,2E1) on ascorbate/iron-induced lipid peroxidation. Using microsomes prepared from human lymphoblastic cells enriched in recombinant cytochrome P450 isoenzymes, we have shown that the degree of peroxidation is a function of the amount of P450 present rather than the presence of any specific isoenzyme. Incorporated P450 increased the amount of peroxidation products by up to 2.1-fold compared to the control microsomes with no P450. It is therefore concluded that cytochrome P450's play a significant role in ascorbate/iron peroxidation
AB - We have examined the effect of human cytochrome P450's (1A1,1A2,3A4,2A6,2B6,2D6,2E1) on ascorbate/iron-induced lipid peroxidation. Using microsomes prepared from human lymphoblastic cells enriched in recombinant cytochrome P450 isoenzymes, we have shown that the degree of peroxidation is a function of the amount of P450 present rather than the presence of any specific isoenzyme. Incorporated P450 increased the amount of peroxidation products by up to 2.1-fold compared to the control microsomes with no P450. It is therefore concluded that cytochrome P450's play a significant role in ascorbate/iron peroxidation
U2 - 10.3109/10715769609088015
DO - 10.3109/10715769609088015
M3 - Article
SN - 1071-5762
VL - 24
SP - 177
EP - 185
JO - Free Radical Research
JF - Free Radical Research
IS - 3
ER -