Hydrophilic Interaction Ultra-High Performance Liquid Chromatography Coupled with Triple-Quadrupole Mass Spectrometry for Determination of Nucleosides and Nucleobases in Animal Horns

Rui Lui, Jin-Ao Duan, Chuan Chai, Hongmei Wen, Sheng Guo, Xinzhi Wang, Yang Zhang, Hao Wu, Mei Zhou, Tianbao Chen, Christopher Shaw

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Traditional Chinese Medicines (TCMs) derived from animal horns are one of the most important types of Chinese medicine. In the present study, a fast and sensitive analytical method was established for qualitative and quantitative determination of 14 nucleosides and nucleobases in animal horns using hydrophilic interaction ultra-high performance liquid chromatography coupled with triple-quadruple tandem mass spectrometry (HILIC-UPLC-QQQ-MS/MS) in selective reaction monitoring (SRM) mode. The method was optimized and validated, and showed good linearity, precision, repeatability, and accuracy. The method was successfully used to determine contents of the 14 nucleosides and nucleobases in 25 animal horn samples. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) were performed and the 25 samples were thereby divided into two groups, which agreed with taxonomy. The method may enable quick and effective search of substitutes for precious horns.
Original languageEnglish
Pages (from-to)1185-1193
Number of pages9
JournalJournal of Liquid Chromatography & Related Technologies
Volume38
Issue number12
Early online date18 Mar 2015
DOIs
Publication statusPublished - 2015

Keywords

  • Animal horns; Hydrophilic interaction chromatography; LC-MS/MS; Nucleobases; Nucleosides

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