Identification and bioactivity evaluation of a novel bradykinin inhibitory peptide from the skin secretion of Chinese large odorous frog, Odorrana livida

Kundi Yang, Chengbang Ma, Mei Zhou, Lei Wang, Renjie Li, Tianbao Chen, Christopher Shaw, W. Li

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A novel peptide was isolated from the skin secretion of Chinese large odorous frog, Odorrana livida, and was named as Rana-BI. The cDNA sequencing was obtained by 'shotgun' cloning. The amino acid sequence of the mature peptide was identified as Gly-Leu-Leu-Ser-Gly-Lys-Ser-Val-Lys-Gly-Ser-Ile-OH by automated Edman degradation, and the molecular weight of the peptide was confirmed to be 1144.68 Da by MALDI-TOF and liquid chromatography/MS. Subsequently, the bioactivity of synthetic peptide was evaluated by smooth muscle assay using isolated rat bladder preparation. It was demonstrated that Rana-BI inhibited the contraction of rat bladder induced by bradykinin. Comparing with other peptides by searching from database, the primary structure of Rana-BI showed high similarity with that of an antimicrobial peptide of Rana family (12/12 residues). These data revealed a novel biological function of this peptide
Original languageEnglish
Pages (from-to)181-185
Number of pages5
JournalJournal of peptide science
Volume22
Issue number3
Early online date09 Feb 2016
DOIs
Publication statusPublished - Mar 2016

Keywords

  • Odorrana livida; Rana-BI; bradykinin; sequence; skin secretion

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