Identification and characterization of 10 polymorphic microsatellite loci in the German cockroach, Blattella germanica

W. Booth; S.M. Bogdanowicz; Paulo Prodöhl; R.G. Harrison; C. Schal; E.L. Vargo

doi:10.1111/j.1471-8286.2006.01663.x

Identification and characterization of 10 polymorphic microsatellite loci in the German cockroach, Blattella germanica

W. Booth, S.M. Bogdanowicz, Paulo Prodöhl, R.G. Harrison, C. Schal, E.L. Vargo

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Primer sequences and initial characterization are presented for 10 microsatellite loci isolated from the German cockroach, Blattella germanica. In a sample of 30 individuals from a single population sample, all loci were polymorphic with two to 12 alleles segregating per locus and levels of observed heterozygosity ranging from 0.27 to 0.92. One locus showed a deficit of heterozygotes. Experimental conditions are described for polymerase chain reaction multiplexing, which enables the genotyping of eight loci in three electrophoretic runs consisting of one set of three and two sets of two markers. Seven primer sets cross-amplify in the related Blattella asahinai.

Original language	English
Pages (from-to)	648-650
Number of pages	3
Journal	MOLECULAR ECOLOGY NOTES
Volume	7(4)
Issue number	4
DOIs	https://doi.org/10.1111/j.1471-8286.2006.01663.x
Publication status	Published - Dec 2007

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
Biochemistry
Ecology

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title = "Identification and characterization of 10 polymorphic microsatellite loci in the German cockroach, Blattella germanica",

abstract = "Primer sequences and initial characterization are presented for 10 microsatellite loci isolated from the German cockroach, Blattella germanica. In a sample of 30 individuals from a single population sample, all loci were polymorphic with two to 12 alleles segregating per locus and levels of observed heterozygosity ranging from 0.27 to 0.92. One locus showed a deficit of heterozygotes. Experimental conditions are described for polymerase chain reaction multiplexing, which enables the genotyping of eight loci in three electrophoretic runs consisting of one set of three and two sets of two markers. Seven primer sets cross-amplify in the related Blattella asahinai.",

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T1 - Identification and characterization of 10 polymorphic microsatellite loci in the German cockroach, Blattella germanica

AU - Booth, W.

AU - Bogdanowicz, S.M.

AU - Prodöhl, Paulo

AU - Harrison, R.G.

AU - Schal, C.

AU - Vargo, E.L.

PY - 2007/12

Y1 - 2007/12

N2 - Primer sequences and initial characterization are presented for 10 microsatellite loci isolated from the German cockroach, Blattella germanica. In a sample of 30 individuals from a single population sample, all loci were polymorphic with two to 12 alleles segregating per locus and levels of observed heterozygosity ranging from 0.27 to 0.92. One locus showed a deficit of heterozygotes. Experimental conditions are described for polymerase chain reaction multiplexing, which enables the genotyping of eight loci in three electrophoretic runs consisting of one set of three and two sets of two markers. Seven primer sets cross-amplify in the related Blattella asahinai.

AB - Primer sequences and initial characterization are presented for 10 microsatellite loci isolated from the German cockroach, Blattella germanica. In a sample of 30 individuals from a single population sample, all loci were polymorphic with two to 12 alleles segregating per locus and levels of observed heterozygosity ranging from 0.27 to 0.92. One locus showed a deficit of heterozygotes. Experimental conditions are described for polymerase chain reaction multiplexing, which enables the genotyping of eight loci in three electrophoretic runs consisting of one set of three and two sets of two markers. Seven primer sets cross-amplify in the related Blattella asahinai.

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JO - MOLECULAR ECOLOGY NOTES

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IS - 4

ER -

Identification and characterization of 10 polymorphic microsatellite loci in the German cockroach, Blattella germanica

Abstract

ASJC Scopus subject areas

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