Identification and characterization of 10 polymorphic microsatellite loci in the German cockroach, Blattella germanica

W. Booth, S.M. Bogdanowicz, Paulo Prodöhl, R.G. Harrison, C. Schal, E.L. Vargo

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Primer sequences and initial characterization are presented for 10 microsatellite loci isolated from the German cockroach, Blattella germanica. In a sample of 30 individuals from a single population sample, all loci were polymorphic with two to 12 alleles segregating per locus and levels of observed heterozygosity ranging from 0.27 to 0.92. One locus showed a deficit of heterozygotes. Experimental conditions are described for polymerase chain reaction multiplexing, which enables the genotyping of eight loci in three electrophoretic runs consisting of one set of three and two sets of two markers. Seven primer sets cross-amplify in the related Blattella asahinai.
Original languageEnglish
Pages (from-to)648-650
Number of pages3
JournalMOLECULAR ECOLOGY NOTES
Volume7(4)
Issue number4
DOIs
Publication statusPublished - Dec 2007

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Ecology

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