Identification and functional analysis of a novel bradykinin inhibitory peptide in the venoms of New World Crotalinae pit vipers.

R.L.J. Graham, C. Graham, S. McClean, Tianbao Chen, M. O'Rourke, David Hirst, D. Theakston, Christopher Shaw

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)


A novel undecapeptide has been isolated and structurally characterized from the venoms of three species of New World pit vipers from the subfamily, Crotalinae. These include the Mexican moccasin (Agkistrodon bilineatus), the prairie rattlesnake (Crotalus viridis viridis), and the South American bushmaster (Lachesis muta). The peptide was purified from all three venoms using a combination of gel permeation chromatography and reverse-phase HPLC. Automated Edman degradation sequencing and MALDI-TOF mass spectrometry established its peptide primary structure as: Thr-Pro-Pro-Ala-Gly-Pro-Asp-Val-Gly-Pro-Arg-OH, with a non-protonated molecular mass of 1063.18 Da. A synthetic replicate of the peptide was found to be an antagonist of bradykinin action at the rat vascular B2 receptor. This is the first bradykinin inhibitory peptide isolated from snake venom. Database searching revealed the peptide to be highly structurally related (10/11 residues) with a domain residing between the bradykinin-potentiating peptide and C-type natriuretic peptide domains of a recently cloned precursor from tropical rattlesnake (Crotalus durissus terrificus) venom gland. BIP thus represents a novel biological entity from snake venom.
Original languageEnglish
Pages (from-to)1587-1592
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 23 Dec 2005


  • Amino Acid Sequence
  • Animals
  • Bradykinin/antagonists & inhibitors
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Crotalid Venoms/chemistry
  • Male
  • Molecular Sequence Data
  • Peptides/chemistry
  • Rats
  • Rats, Wistar
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Tail/drug effects
  • Viperidae

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


Dive into the research topics of 'Identification and functional analysis of a novel bradykinin inhibitory peptide in the venoms of New World Crotalinae pit vipers.'. Together they form a unique fingerprint.

Cite this