Identification of a general secretory pathway in a human isolate of Burkholderia vietnamiensis (formerly B. cepacia complex genomovar V) that is required for the secretion of hemolysin and phospholipase C activities

Christine C Fehlner-Gardiner, Tammy M-H Hopkins, Miguel A Valvano

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Members of the Burkholderia cepacia complex can secrete proteases, lipases, and hemolysins. We report in this study the identification of a general secretory pathway present in a B. vietnamiensis (formerly genomovar V) clinical isolate, which is required for the efficient secretion of phospholipase C and hemolysin activities. Southern blot hybridization experiments revealed that this general secretion pathway is highly conserved among the different genomovars of the B. cepacia complex and is homologous to a similar system described in B. pseudomallei. We also show that this pathway appears not to be necessary for intracellular survival of B. vietnamiensis within Acanthamoeba polyphaga.
Original languageEnglish
Pages (from-to)249-54
Number of pages6
JournalMicrobial Pathogenesis
Volume32
Issue number5
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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