Identification of a general secretory pathway in a human isolate of Burkholderia vietnamiensis (formerly B. cepacia complex genomovar V) that is required for the secretion of hemolysin and phospholipase C activities

Christine C Fehlner-Gardiner; Tammy M-H Hopkins; Miguel A Valvano

doi:10.1006/mpat.2002.0503

Identification of a general secretory pathway in a human isolate of Burkholderia vietnamiensis (formerly B. cepacia complex genomovar V) that is required for the secretion of hemolysin and phospholipase C activities

Christine C Fehlner-Gardiner
, Tammy M-H Hopkins
, Miguel A Valvano

School of Medicine, Dentistry and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Members of the Burkholderia cepacia complex can secrete proteases, lipases, and hemolysins. We report in this study the identification of a general secretory pathway present in a B. vietnamiensis (formerly genomovar V) clinical isolate, which is required for the efficient secretion of phospholipase C and hemolysin activities. Southern blot hybridization experiments revealed that this general secretion pathway is highly conserved among the different genomovars of the B. cepacia complex and is homologous to a similar system described in B. pseudomallei. We also show that this pathway appears not to be necessary for intracellular survival of B. vietnamiensis within Acanthamoeba polyphaga.

Original language	English
Pages (from-to)	249-54
Number of pages	6
Journal	Microbial Pathogenesis
Volume	32
Issue number	5
DOIs	https://doi.org/10.1006/mpat.2002.0503
Publication status	Published - 2002

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Microbiology
Infectious Diseases

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10.1006/mpat.2002.0503

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title = "Identification of a general secretory pathway in a human isolate of Burkholderia vietnamiensis (formerly B. cepacia complex genomovar V) that is required for the secretion of hemolysin and phospholipase C activities",

abstract = "Members of the Burkholderia cepacia complex can secrete proteases, lipases, and hemolysins. We report in this study the identification of a general secretory pathway present in a B. vietnamiensis (formerly genomovar V) clinical isolate, which is required for the efficient secretion of phospholipase C and hemolysin activities. Southern blot hybridization experiments revealed that this general secretion pathway is highly conserved among the different genomovars of the B. cepacia complex and is homologous to a similar system described in B. pseudomallei. We also show that this pathway appears not to be necessary for intracellular survival of B. vietnamiensis within Acanthamoeba polyphaga.",

author = "Fehlner-Gardiner, \{Christine C\} and Hopkins, \{Tammy M-H\} and Valvano, \{Miguel A\}",

year = "2002",

doi = "10.1006/mpat.2002.0503",

language = "English",

volume = "32",

pages = "249--54",

journal = "Microbial Pathogenesis",

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Identification of a general secretory pathway in a human isolate of Burkholderia vietnamiensis (formerly B. cepacia complex genomovar V) that is required for the secretion of hemolysin and phospholipase C activities. / Fehlner-Gardiner, Christine C; Hopkins, Tammy M-H; Valvano, Miguel A.
In: Microbial Pathogenesis, Vol. 32, No. 5, 2002, p. 249-54.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Hopkins, Tammy M-H

AU - Valvano, Miguel A

PY - 2002

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AB - Members of the Burkholderia cepacia complex can secrete proteases, lipases, and hemolysins. We report in this study the identification of a general secretory pathway present in a B. vietnamiensis (formerly genomovar V) clinical isolate, which is required for the efficient secretion of phospholipase C and hemolysin activities. Southern blot hybridization experiments revealed that this general secretion pathway is highly conserved among the different genomovars of the B. cepacia complex and is homologous to a similar system described in B. pseudomallei. We also show that this pathway appears not to be necessary for intracellular survival of B. vietnamiensis within Acanthamoeba polyphaga.

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DO - 10.1006/mpat.2002.0503

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JO - Microbial Pathogenesis

JF - Microbial Pathogenesis

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