Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells

B. Warth, G. Del Favero, G. Wiesenberger, H. Puntscher, L. Woelflingseder, P. Fruhmann, B. Sarkanj, R. Krska, R. Schuhmacher, G. Adam, D. Marko

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Abstract

The mycotoxin deoxynivalenol (DON) is an abundant contaminant of cereal based food and a severe issue for global food safety. We report the discovery of DON-3-sulfate as a novel human metabolite and potential new biomarker of DON exposure. The conjugate was detectable in 70% of urine samples obtained from pregnant women in Croatia. For the measurement of urinary metabolites, a highly sensitive and selective LC-MS/MS method was developed and validated. The method was also used to investigate samples from a duplicate diet survey for studying the toxicokinetics of DON-3-sulfate. To get a preliminary insight into the biological relevance of the newly discovered DON-sulfates, in vitroexperiments were performed. In contrast to DON, sulfate conjugates lacked potency to suppress protein translation. However, surprisingly we found that DON-sulfates enhanced proliferation of human HT-29 colon carcinoma cells, primary human colon epithelial cells (HCEC-1CT) and, to some extent, also T24 bladder cancer cells. A proliferative stimulus, especially in tumorigenic cells raises concern on the potential impact of DON-sulfates on consumer health. Thus, a further characterization of their toxicological relevance should be of high priority. © The Author(s) 2016.
Original languageEnglish
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 23 Sep 2016

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