Identification of a suitable sterilisation method for collagen derived from a marine Demosponge

Iwan Palmer, Susan A. Clarke, John Nelson, Wolfgang Schatton, Nicholas J. Dunne, Fraser Buchanan

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
1554 Downloads (Pure)


Collagen is widely used as a biomedical material, and its importance is likely to grow as research and understanding progresses in this field. As a biomedical material, ensuring the sterility of collagen before use as, or incorporation into, a medical device is paramount. However, common sterilisation techniques can induce changes in the physical structure and protein chemistry of collagen, potentially affecting the performance. In this preliminary study, the influence of autoclaving, gamma irradiation and ethylene oxide gas sterilisation on the denaturation temperature and helical content of the collagen was evaluated using differential scanning calorimetry and Fourier transform infrared spectroscopy. Early results indicate that all sterilisation techniques affect collagen properties but suggest that the least damaging of the techniques investigated was y irradiation.
Original languageEnglish
Pages (from-to)148-163
Number of pages16
JournalInternational Journal of Nano and Biomaterials
Issue number2
Publication statusPublished - 2012


  • sterilisation

ASJC Scopus subject areas

  • Biomaterials
  • Physical and Theoretical Chemistry
  • Biomedical Engineering
  • Atomic and Molecular Physics, and Optics


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