Identifying an emergent adulterant hydrochlorothiazide in food: A simple lateral flow strip with high sensitivity by time-resolved fluorescence

Runlin Shen, Tian Guan, Zhaodong Li, Ziling Hong, Boris B. Dzantiev, Anatoly V. Zherdev, Anastasios Koidis, Xiaojun Yao*, Hongtao Lei*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
45 Downloads (Pure)

Abstract

Hydrochlorothiazide, an antihypertensive and diuretic drug only for clinic purpose, was recently found as an adulterant in functional foods. Due to the potential health risk, it is urgent to develop a simple and rapid assay for its identification. In this work, two hapten structures were rationally designed based on molecular modeling, and a highly sensitive antibody to hydrochlorothiazide was obtained with 50% inhibitive concentration (IC50) of 6.7 ng/mL for the first time. A lateral flow strip assay was then developed based on time resolved fluorescence, demonstrating a cut-off value of 250 μg/kg to hydrochlorothiazide in functional capsules and tablets, and a negligible cross-reactivity to related compounds. The strip performance for real samples collected from the retail markets was consistent of that of confirmed method (LC-MS/MS). As an ideal tool, the proposed strip could be applied to identify adulterated hydrochlorothiazide in functional foods.

Original languageEnglish
Article number109265
JournalFood Control
Volume143
Early online date11 Aug 2022
DOIs
Publication statusPublished - Jan 2023

Bibliographical note

Funding Information:
This work was supported financially by the Natural Science Foundation of China ( 31871883 ), the National Key Research and Development Program of Thirteenth Five-Year Plan (No. 2017YFC1601700 ), Generic Technique Innovation Team Construction of Modern Agriculture of Guangdong Province ( 2021KJ130 ), HeYuan Planned Program in Science and Technology ( 210115091474673 ).

Publisher Copyright:
© 2022 Elsevier Ltd

Keywords

  • Adulterant
  • Antibody
  • Functional food
  • Hydrochlorothiazide
  • Immunochromatography
  • Strip
  • Time-resolved fluorescence

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

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