Imaging glucoamylase by scanning tunnelling microscopy

A. Patrick Gunning; Victor J.  Morris; Gary Williamson; Nigel J Belshaw; Gerard F. H.  Kramer; Marja W.  Kanning

doi:10.1039/AN9941901939

Imaging glucoamylase by scanning tunnelling microscopy

A. Patrick Gunning
, Victor J. Morris
, Gary Williamson
, Nigel J Belshaw
, Gerard F. H. Kramer
, Marja W. Kanning

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.

Original language	English
Pages (from-to)	1939-1942
Number of pages	4
Journal	Analyst
Volume	119
Issue number	9
DOIs	https://doi.org/10.1039/AN9941901939
Publication status	Published - 01 Sept 1994
Externally published	Yes

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title = "Imaging glucoamylase by scanning tunnelling microscopy",

abstract = "Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.",

author = "Gunning, \{A. Patrick\} and Morris, \{Victor J.\} and Gary Williamson and Belshaw, \{Nigel J\} and Kramer, \{Gerard F. H.\} and Kanning, \{Marja W.\}",

year = "1994",

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doi = "10.1039/AN9941901939",

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T1 - Imaging glucoamylase by scanning tunnelling microscopy

AU - Gunning, A. Patrick

AU - Morris, Victor J.

AU - Williamson, Gary

AU - Belshaw, Nigel J

AU - Kramer, Gerard F. H.

AU - Kanning, Marja W.

PY - 1994/9/1

Y1 - 1994/9/1

N2 - Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.

AB - Images of the enzymes glucoamylase 1 and glucoamylase 2 and G1C (a proteolytically derived C-terminal fragment of glucoamylase 1) have been obtained by scanning tunnelling microscopy (STM). The images of glucoamylase 2 and G1C show that these proteins are nearly spherical globular proteins. In contrast, images of glucoamylase 1 show that it is a dumb-bell shaped globular protein. Problems encountered in (i) imaging these proteins and (ii) validating and interpreting the images obtained by STM are discussed.

U2 - 10.1039/AN9941901939

DO - 10.1039/AN9941901939

M3 - Article

SN - 0003-2654

VL - 119

SP - 1939

EP - 1942

JO - Analyst

JF - Analyst

IS - 9

ER -

Imaging glucoamylase by scanning tunnelling microscopy

Abstract

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