Immune response modulation by HPV16 oncoproteins in lung cancer: insights from clinical and in vitro investigations

Bianca de França São Marcos; Daffany Luana dos Santos; Georon Ferreira de Sousa; Leonardo Carvalho de Oliveira Cruz; Bárbara Rafaela da Silva Barros; Matheus Gardini Amâncio Marques de Sena; Vanessa Emanuelle Pereira Santos; Talita Helena de Araújo Oliveira; Cristiane Moutinho Lagos de Melo; Antonio Carlos de Freitas

doi:10.3390/v16111731

Immune response modulation by HPV16 oncoproteins in lung cancer: insights from clinical and in vitro investigations

Bianca de França São Marcos, Daffany Luana dos Santos, Georon Ferreira de Sousa, Leonardo Carvalho de Oliveira Cruz, Bárbara Rafaela da Silva Barros, Matheus Gardini Amâncio Marques de Sena, Vanessa Emanuelle Pereira Santos, Talita Helena de Araújo Oliveira, Cristiane Moutinho Lagos de Melo, Antonio Carlos de Freitas

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Abstract

Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes’ expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients’ samples. In the in vitro analysis, a decrease in the number of monocytes and cytotoxic cells was observed when co-stimulated by E6 and E7, and it promoted an increase in the Th2 profile. In contrast, the high proliferation of cytotoxic cells in A549 cells transfected with E5, associated with the high expression of costimulatory molecules in monocytes, suggests a low capacity of E5 to inhibit the presentation of antigens by antigen-presenting cells (APC) and a possible use of E5 in future therapeutic strategies against lung cancers associated with HPV.

Original language	English
Article number	1731
Journal	Viruses
Volume	16
Issue number	11
Early online date	04 Nov 2024
DOIs	https://doi.org/10.3390/v16111731
Publication status	Published - 04 Nov 2024

Keywords

Immune response
Immune response modulation
HPV16 oncoproteins
lung cancer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/v16111731Licence: CC BY

Immune response modulation by HPV16 oncoproteins in lung cancer: insights from clinical and in vitro investigations
Copyright 2024 the authors. This is an open access article published under a Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Final published version, 3.01 MBLicence: CC BY

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São Marcos, B. D. F., Santos, D. L. D., Sousa, G. F. D., Cruz, L. C. D. O., Barros, B. R. D. S., de Sena, M. G. A. M., Santos, V. E. P., Oliveira, T. H. D. A., Lagos de Melo, C. M., & de Freitas, A. C. (2024). Immune response modulation by HPV16 oncoproteins in lung cancer: insights from clinical and in vitro investigations. Viruses, 16(11), Article 1731. https://doi.org/10.3390/v16111731

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abstract = "Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes{\textquoteright} expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients{\textquoteright} samples. In the in vitro analysis, a decrease in the number of monocytes and cytotoxic cells was observed when co-stimulated by E6 and E7, and it promoted an increase in the Th2 profile. In contrast, the high proliferation of cytotoxic cells in A549 cells transfected with E5, associated with the high expression of costimulatory molecules in monocytes, suggests a low capacity of E5 to inhibit the presentation of antigens by antigen-presenting cells (APC) and a possible use of E5 in future therapeutic strategies against lung cancers associated with HPV.",

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AU - São Marcos, Bianca de França

AU - Santos, Daffany Luana dos

AU - Sousa, Georon Ferreira de

AU - Cruz, Leonardo Carvalho de Oliveira

AU - Barros, Bárbara Rafaela da Silva

AU - de Sena, Matheus Gardini Amâncio Marques

AU - Santos, Vanessa Emanuelle Pereira

AU - Oliveira, Talita Helena de Araújo

AU - Lagos de Melo, Cristiane Moutinho

AU - de Freitas, Antonio Carlos

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N2 - Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes’ expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients’ samples. In the in vitro analysis, a decrease in the number of monocytes and cytotoxic cells was observed when co-stimulated by E6 and E7, and it promoted an increase in the Th2 profile. In contrast, the high proliferation of cytotoxic cells in A549 cells transfected with E5, associated with the high expression of costimulatory molecules in monocytes, suggests a low capacity of E5 to inhibit the presentation of antigens by antigen-presenting cells (APC) and a possible use of E5 in future therapeutic strategies against lung cancers associated with HPV.

AB - Lung cancer has the highest mortality rates worldwide, and Human Papillomavirus (HPV) has been associated with its carcinogenesis. In this study, HPV16 genes’ expressions were investigated in patient samples, along with the immunological response promoted by lymphocytes and monocytes in A549 cells transfected with HPV oncogenes and co-cultured with PBMC. An increase in the expression of E5 was observed in the patients’ samples. In the in vitro analysis, a decrease in the number of monocytes and cytotoxic cells was observed when co-stimulated by E6 and E7, and it promoted an increase in the Th2 profile. In contrast, the high proliferation of cytotoxic cells in A549 cells transfected with E5, associated with the high expression of costimulatory molecules in monocytes, suggests a low capacity of E5 to inhibit the presentation of antigens by antigen-presenting cells (APC) and a possible use of E5 in future therapeutic strategies against lung cancers associated with HPV.

KW - Immune response

KW - Immune response modulation

KW - HPV16 oncoproteins

KW - lung cancer

U2 - 10.3390/v16111731

DO - 10.3390/v16111731

M3 - Article

SN - 1999-4915

VL - 16

JO - Viruses

JF - Viruses

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M1 - 1731

ER -

Immune response modulation by HPV16 oncoproteins in lung cancer: insights from clinical and in vitro investigations

Abstract

Keywords

UN SDGs

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